Ogbe F O, Thottappilly G, Dixon A G O, Atiri G I, Mignouna H D
International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria and National Root Crops Research Institute, Umudike, Umuahia, Nigeria.
IITA and Mahyco Research Foundation, Hyderabad, India.
Plant Dis. 2003 Mar;87(3):229-232. doi: 10.1094/PDIS.2003.87.3.229.
In a survey for cassava mosaic begomoviruses conducted in 1997 and 1998 in Nigeria, East African cassava mosaic virus (EACMV) was detected by the polymerase chain reaction together with African cassava mosaic virus (ACMV) in 27 out of 290 cassava leaf samples of infected plants from 254 farmers' fields in five agroecological zones. One plant was infected with EACMV only. Five variant isolates of EACMV were observed based on their reactions to primers that could detect Cameroonian and East African strains of EACMV. Isolates of variants 1 and 3 occurred mostly in the derived or coastal and southern Guinea savannahs, while variants 4 and 5 predominated in the humid forest region. Isolates of variant 2 were widely distributed across the three agroecologies. EACMV was not detected in the northern Guinea savannah and arid and semiarid zones. Most doubly infected plants showed more severe symptoms than plants with single infection. Occurrence of EACMV variants together with ACMV detection and information about their distribution in Nigeria could be used for the selection of cassava clones in cassava mosaic disease resistance programs.
1997年和1998年在尼日利亚开展了一项针对木薯花叶贝古莫病毒的调查，通过聚合酶链反应在来自五个农业生态区254个农户田地的290份受感染木薯植株的叶片样本中，检测到东非木薯花叶病毒（EACMV）以及非洲木薯花叶病毒（ACMV），其中27份样本呈阳性。有一株植物仅感染了EACMV。根据对可检测喀麦隆和东非EACMV株系的引物的反应，观察到了EACMV的五个变异分离株。变异株1和3的分离株主要出现在衍生区或沿海以及南部几内亚稀树草原，而变异株4和5在湿润森林地区占主导地位。变异株2的分离株广泛分布于这三种农业生态区。在北部几内亚稀树草原以及干旱和半干旱地区未检测到EACMV。大多数双重感染的植株比单一感染的植株表现出更严重的症状。EACMV变异株的出现以及ACMV的检测结果及其在尼日利亚的分布信息，可用于木薯花叶病抗性计划中木薯克隆品种的选择。
