Analysis of Anti-toxoplasma Immunoglobulin G and Immunoglobulin M Antibody Levels after Intervention with Extract on Early Pregnant Mice with Acute Toxoplasmosis.

BACKGROUND

has strong anti-inflammatory effect. This study aims to evaluated the level of anti-Toxoplasma immunoglobulin G and immunoglobulin M (IgG-IgM) antibody after intervention with extract in early pregnant mice with acute toxoplasmosis.

MATERIALS AND METHODS

We evaluated 20 early pregnant mice that were divided into five groups, four mice in each. Group 1-4 received injections of tachyzoites. Three days later, G1 and G2 were given orally 125 mg/kg/day and 500 mg/kg/day of extract, respectively. G3 was given 60 mg/kg/day of spiramycin (positive control), and G4 was given 0.2 ml of distilled water (negative control). G5 underwent no intervention at all. Blood samples were obtained serially (before and 3 days after injection of tachyzoites, 3 days and 7 days after intervention) to assess anti-Toxoplasma IgG-IgM antibody levels by enzyme-linked immunosorbent assay methods.

RESULTS

Anti-Toxoplasma IgG-IgM antibody levels increased significantly 3 days after injection of tachyzoites ( < 0.05), but decreased significantly ( < 0.05) 3 days, and 7 days after administration of extract dose 125 mg, 500 mg, and spiramycin 60 mg, and there was no significant difference between these three groups. Anti-Toxoplasma IgG-IgM antibody levels increased significantly ( < 0.05) 3 days, 6 days, and 10 days after injections of tachyzoites on G4. The IgG-IgM antibody levels fluctuated on G5 and considered as insignificant ( > 0.05).

CONCLUSION

The administration of extract at a dose of 125 mg/kg/day for 7 days effectively decreased anti-Toxoplasma IgG-IgM antibody level in early pregnant mice with acute toxoplasmosis.