Guo Yan-Zi, He Ping, Feng Ai-Min
The Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi Province, China.
The Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi Province, China.
Asian Pac J Trop Med. 2017 Feb;10(2):175-178. doi: 10.1016/j.apjtm.2017.01.004. Epub 2017 Jan 20.
To observe the effect of curcumin on expressions of nuclear transcription factor-kappa Bp65 (NF-κBp65), TNF-α and IL-8 in placental tissue of premature birth of infected mice induced by lipopolysaccharide (LPS).
A total of 60 C57BL/6 mice pregnant with 15 d were collected and randomly divided into control group, model group, treatment group and preventative group. LPS was repeatedly injected in abdominal cavity to construct infected premature birth model, while mice of control group were given with 100 mg/kg of vitamin C through abdominal cavity injection and mice of treatment group and preventative group were given curcumin of 100 mg/kg through abdominal cavity injection after modeling operation and before 1 d of modeling operation, respectively. A total of 5 mice of four groups respectively were executed by cervical dislocation after 6 h, 12 h and 24 h after constructing model. Placental tissues were collected and the immunohistochemical method SABC of immunologic tissue was used to detect the expression of NF-κBp65, TNF-α and IL-8 and peripheral blood of executed mice after 24 h was collected to detect the concentrations of IL-8, malondialdehyde (MDA) and superoxide dismutase (SOD), meanwhile live birth rate of four groups was contrasted.
Staining intensity of NF-κBp65, TNF-α and IL-8 in placental tissue of treatment group and preventative group was significantly higher than control group but lower than model group (P < 0.05). Level of serum IL-8 and MDA of control group was significantly lower than the other three groups (P < 0.05) and level of blood of SOD in model group was significantly lower than control group (P < 0.05). Levels of serum IL-8 and MDA of treatment group and preventative group were significantly lower than model group (P < 0.05) while level of SOD was significantly higher than model group (P < 0.05). Live birth rate of treatment group and preventative group was significantly higher than model group (P < 0.05).
Curcumin can effectively prevent the active pathway of NF-κB in pregnant tissue of premature birth of infected mice, reduce the expression of TNF-α and IL-8 and relieve the damage of lipid peroxide of oxidative stress of LPS on mother-fetus and further to achieve the objective of preventing and curing premature birth induced with infection.
观察姜黄素对脂多糖（LPS）诱导的感染性早产小鼠胎盘组织中核转录因子-κBp65（NF-κBp65）、肿瘤坏死因子-α（TNF-α）和白细胞介素-8（IL-8）表达的影响。
选取60只孕15天的C57BL/6小鼠，随机分为对照组、模型组、治疗组和预防组。通过腹腔反复注射LPS构建感染性早产模型，对照组小鼠腹腔注射100mg/kg维生素C，治疗组和预防组小鼠分别在造模术后和造模前1天腹腔注射100mg/kg姜黄素。造模后6小时、12小时和24小时分别处死每组5只小鼠。收集胎盘组织，采用免疫组织化学SABC法检测NF-κBp65、TNF-α和IL-8的表达，并收集处死小鼠24小时后的外周血检测IL-8、丙二醛（MDA）和超氧化物歧化酶（SOD）的浓度，同时对比四组的活产率。
治疗组和预防组胎盘组织中NF-κBp65、TNF-α和IL-8的染色强度显著高于对照组，但低于模型组（P<0.05）。对照组血清IL-8和MDA水平显著低于其他三组（P<0.05），模型组血液SOD水平显著低于对照组（P<0.05）。治疗组和预防组血清IL-8和MDA水平显著低于模型组（P<0.05），而SOD水平显著高于模型组（P<0.05）。治疗组和预防组的活产率显著高于模型组（P<0.05）。
姜黄素可有效阻断感染性早产小鼠妊娠组织中NF-κB的激活途径，降低TNF-α和IL-8的表达，减轻LPS氧化应激对母胎脂质过氧化的损伤，进而达到防治感染性早产的目的。
