Mişe Yonar S, Yonar M E, Ural M Ş
Department of Aquaculture and Fish Diseases, Firat University, Fisheries Faculty, Elazig, Turkey.
College of Keban, Fisheries Programme, Firat University, Elazig, Turkey.
Cell Mol Biol (Noisy-le-grand). 2017 Mar 31;63(3):68-72. doi: 10.14715/cmb/2017.63.3.13.
The aim of this study was to determine protective effects of curcumin on oxidant/antioxidant parameters in Cyprinus carpio exposed to malathion. The fish were exposed to two sublethal concentrations of malathion (0.5 and 1 mg/L), and curcumin (100 mg per kg of fish weight) was simultaneously administered for 14 days. Malondialdehyde level and superoxide dismutase, catalase, glutathione peroxidase, and glutathione-S-transferase activities were monitored in liver, kidney and gills, which were collected at the end of the experiment. The results revealed a significant increase in the malondialdehyde levels of the groups that were exposed to malathion. Also, malathion exposure caused a significant increase in superoxide dismutase, catalase, and glutathione-S-transferase activities and a significant decrease in glutathione peroxidase activity. Treatment with curcumin attenuated the malathion-induced oxidative stress by significantly decreasing the levels of malondialdehyde in the tissues. In addition, curcumin reversed the superoxide dismutase, catalase, glutathione peroxidase, and glutathione-S-transferase activities. In conclusion, this study demonstrated that malathion caused oxidative stress and negative alterations on the antioxidant enzyme activities of the fish. However, this toxic effect was neutralised by the administration of curcumin.
本研究的目的是确定姜黄素对暴露于马拉硫磷的鲤鱼体内氧化/抗氧化参数的保护作用。将鱼暴露于两种亚致死浓度的马拉硫磷（0.5和1毫克/升），同时给予姜黄素（每千克鱼体重100毫克），持续14天。在实验结束时收集肝脏、肾脏和鳃，监测丙二醛水平以及超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶的活性。结果显示，暴露于马拉硫磷的组中丙二醛水平显著升高。此外，马拉硫磷暴露导致超氧化物歧化酶、过氧化氢酶和谷胱甘肽-S-转移酶活性显著增加，而谷胱甘肽过氧化物酶活性显著降低。姜黄素处理通过显著降低组织中丙二醛水平减轻了马拉硫磷诱导的氧化应激。此外，姜黄素使超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶的活性恢复正常。总之，本研究表明马拉硫磷会导致鱼类氧化应激并对抗氧化酶活性产生负面改变。然而，这种毒性作用通过给予姜黄素得以中和。
