Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Naunyn Schmiedebergs Arch Pharmacol. 2023 Aug;396(8):1713-1725. doi: 10.1007/s00210-023-02424-6. Epub 2023 Feb 20.
Crocin is the main bioactive components of the saffron which has positive role in the nervous system; however, its neuroprotective activity is not fully elicited. So, the aim of the current study was to determine effects of the crocin on reflexive motor and anti-depressive behaviors as well as serum and brain tissue antioxidant activities in cuprizone-induced (CPZ) model of multiple sclerosis (MS) mice. Forty male C57BL/6 mice were randomly assigned into 4 groups. Mice in the control group were received normal diet. In group 2, mice received normal diet and orally received crocin (100 mg/kg) 3 times per week for 5 weeks. In group 3, CPZ-induced demyelination was done by chew palate containing 0.2% (w/w) CPZ for 5 weeks. In group 4, mice feed CPZ containing diet and orally received crocin (100 mg/kg) three times per for 5 weeks. After determination of the MS signs, reflexive motor behavior and depressive tests were done. Also, serum and brain tissue antioxidant activity was determined. According to the data, CPZ had negative effects on hind-limb foot angle, hind- and front-limb suspension, surface righting, grip strength, and negative geotaxis while crocin improved it. Co-administration of the CPZ + crocin reversed effect of the CPZ on the reflexive motor behaviors. CPZ increased immobility time in the forced swimming test (FST) and tail suspension test (TST), while co-administration of the CPZ + crocin reversed effect of the CPZ on immobility time. CPZ decreased number of cross in open field test (OFT) and spending time on rotarod, while co-administration of the CPZ + crocin reversed effect of the CPZ. Malondialdehyde (MDA) production increased, and glutathione peroxidase (GPx), superoxide dismutase (SOD), and total antioxidant status (TAS) levels decreased in serum and brain tissue of the mice treated with CPZ. Pretreatment with crocin decreased adverse effect of the CPZ on serum and brain tissue antioxidants. These results suggested crocin has protective effect against CPZ-induced MS in mice.
西红花中的主要生物活性成分藏红花素对神经系统有积极作用，但它的神经保护活性尚未完全被激发。因此，本研究旨在确定藏红花对杯状病毒诱导的多发性硬化症（MS）模型小鼠反射性运动和抗抑郁行为以及血清和脑组织抗氧化活性的影响。将 40 只雄性 C57BL/6 小鼠随机分为 4 组。对照组小鼠给予正常饮食。第 2 组小鼠给予正常饮食，每周 3 次口服藏红花（100mg/kg），连续 5 周。第 3 组，通过咀嚼含有 0.2%（w/w）CPZ 的食物诱导脱髓鞘，持续 5 周。第 4 组，给予 CPZ 饮食并每周 3 次口服藏红花（100mg/kg），连续 5 周。在确定 MS 症状后，进行反射性运动行为和抑郁测试，并测定血清和脑组织的抗氧化活性。根据数据，CPZ 对后肢足角、后肢和前肢悬挂、表面翻转、握力和负趋地性有负面影响，而藏红花则改善了这些影响。CPZ+藏红花联合给药逆转了 CPZ 对反射性运动行为的影响。CPZ 增加了强迫游泳试验（FST）和悬尾试验（TST）中的不动时间，而 CPZ+藏红花联合给药则逆转了 CPZ 对不动时间的影响。CPZ 减少了开阔场试验（OFT）中的穿越次数和旋转棒上的停留时间，而 CPZ+藏红花联合给药则逆转了 CPZ 的影响。CPZ 导致血清和脑组织中丙二醛（MDA）生成增加，谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）和总抗氧化状态（TAS）水平降低。藏红花预处理降低了 CPZ 对血清和脑组织抗氧化剂的不良影响。这些结果表明，藏红花对 CPZ 诱导的 MS 小鼠具有保护作用。
