Department of Agricultural, Food and Nutritional Science, Center for Health Research Innovation, University of Alberta, 4-002G Li Ka Shing, Edmonton, AB, T6G 2E1, Canada.
STELA Dairy Research Center, Institute of Nutrition and Functional Foods (INAF), Université Laval, Québec, Canada.
Eur J Nutr. 2021 Aug;60(5):2807-2818. doi: 10.1007/s00394-020-02462-3. Epub 2021 Jan 8.
To determine the effect of feeding buttermilk-derived choline metabolites on the immune system development in Sprague-Dawley rat pups.
Sprague-Dawley dams were randomized to one of the three diets containing 1.7 g/kg choline: 1-Control (100% free choline (FC)), 2-Buttermilk (BM, 37% phosphatidylcholine (PC), 34% sphingomyelin (SM), 17% glycerophosphocholine (GPC), 7% FC, 5% phosphocholine), and 3-Placebo (PB, 50% PC, 25% FC, 25% GPC) until the end of the lactation period. At weaning, pups continued on the same diet as their mom. Cell phenotypes and cytokine production by mitogen-stimulated splenocytes isolated from 3- and 10-week-old pups were measured.
At 3 weeks, BM-pups had a higher proportion of cytotoxic T cells (CTL; CD3 + CD8 +) while both BM- and PB-pups had an increased proportion of cells expressing CD28 + , CD86 + and CD27 + (all p > 0.05). Following ConA stimulation, splenocytes from BM- and PB-pups produced more TNF-α and IFN-γ and after LPS stimulation produced more IL-10 and TNF-α (all p > 0.05). Starting at week 6 of age, BM-pups had a higher body weight. At 10 weeks, both the BM- and PB-pups had a higher proportion of CTL expressing CD27 + . After ConA stimulation, splenocytes from BM- and PB-pups produced more IL-2, IFN-γ and IL-6 and more IL-10 after LPS stimulation (all p > 0.05).
The proportion of lipid soluble forms of choline in the diet during lactation and weaning periods influence the immune system development in rat offspring.
确定给食乳衍生胆碱代谢物对斯普拉格-道利大鼠幼仔免疫系统发育的影响。
斯普拉格-道利母鼠被随机分为三组中的一组，每组含有 1.7g/kg 胆碱：1-对照（100%游离胆碱（FC））、2-乳清（BM，37%磷脂酰胆碱（PC）、34%鞘磷脂（SM）、17%甘油磷酸胆碱（GPC）、7%FC、5%磷酸胆碱）和 3-安慰剂（PB，50%PC、25%FC、25%GPC），直至哺乳期结束。断奶时，幼仔继续食用与其母亲相同的饮食。测量来自 3 周和 10 周龄幼仔的脾细胞经有丝分裂原刺激后分离出的细胞表型和细胞因子产生情况。
在 3 周时，BM 幼仔的细胞毒性 T 细胞（CTL；CD3+CD8+）比例更高，而 BM 和 PB 幼仔的 CD28+、CD86+和 CD27+细胞比例均增加（均 p>0.05）。经 ConA 刺激后，BM 和 PB 幼仔的脾细胞产生更多的 TNF-α 和 IFN-γ，经 LPS 刺激后产生更多的 IL-10 和 TNF-α（均 p>0.05）。从 6 周龄开始，BM 幼仔的体重更高。在 10 周时，BM 和 PB 幼仔的 CTL 表达 CD27+的比例更高。经 ConA 刺激后，BM 和 PB 幼仔的脾细胞产生更多的 IL-2、IFN-γ和 IL-6，经 LPS 刺激后产生更多的 IL-10（均 p>0.05）。
哺乳期和断奶期饮食中脂溶性胆碱形式的比例影响大鼠后代免疫系统的发育。
