Key Laboratory of Chem-Biosensing, Anhui province, Key Laboratory of Functional Molecular Solids, Anhui province, College of Chemistry and Materials Science, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, PR China.
Analyst. 2019 Mar 25;144(7):2423-2429. doi: 10.1039/c9an00017h.
Since fluorescence assays with high sensitivity for organophosphorus pesticides (OPs) are urgently required to protect the ecosystem and prevent disease, an environmentally friendly and label-free fluorescent probe is desirable. Herein, a poly-thymine30 DNA-templated copper nanoparticle (poly T30-Cu NPs) hydrogel fluorescent probe was explored for the construction of an OPs sensing platform via tyrosinase (TYR) enzyme-controlled quenching. Initially, TYR can efficiently quench the fluorescence of poly T30-Cu NPs; however, when OPs are mixed with a certain amount of TYR, the fluorescence of poly T30-Cu NPs can be recovered. Based on this phenomenon, we designed a functionalized hydrogel based on poly T30-Cu NPs for portable and visible detection of OPs with high sensitivity and selectivity. This proposed fluorescent platform was demonstrated to enable rapid detection of OPs (paraoxon as the model analyte) and provide excellent sensitivity with a detection limit of 3.33 × 10-5 ng μL-1 and a linear range of 1.0 × 10-4-1.0 ng μL-1. The fluorescent probe does not require a sophisticated synthesis and labeling process; in addition, it is environmentally friendly because of the presence of a biotemplate of DNA and biocompatible copper. Moreover, the functional hydrogel combines the features of portability, visualization, fast signal response and environmental anti-interference that make the proposed strategy more feasible in complex practical detection.
由于需要高灵敏度的荧光测定法来保护生态系统和预防疾病，因此理想的是具有环境友好性且无标记的荧光探针。在此，通过酶（TYR）控制的淬灭作用，探索了一种多胸腺嘧啶 30 DNA 模板化的铜纳米颗粒（poly T30-Cu NPs）水凝胶荧光探针，用于构建 OPs 传感平台。最初，TYR 可以有效地猝灭 poly T30-Cu NPs 的荧光；但是，当将 OPs 与一定量的 TYR 混合时，poly T30-Cu NPs 的荧光可以恢复。基于此现象，我们设计了一种基于 poly T30-Cu NPs 的功能化水凝胶，用于便携式和可见检测 OPs，具有高灵敏度和选择性。该荧光平台可用于快速检测 OPs（以对氧磷作为模型分析物），并具有出色的灵敏度，检测限为 3.33×10-5ng μL-1，线性范围为 1.0×10-4-1.0 ng μL-1。荧光探针不需要复杂的合成和标记过程；此外，由于存在 DNA 生物模板和生物相容性铜，因此它是环保的。此外，功能水凝胶结合了便携性、可视化、快速信号响应和环境抗干扰的特点，使得所提出的策略在复杂的实际检测中更具可行性。
