Gowen M, Mundy G R
J Immunol. 1986 Apr 1;136(7):2478-82.
Human peripheral blood cells, when cultured in vitro, release bone-resorbing factors, which have been called osteoclast-activating factors (OAF) but remain unidentified. We showed previously that a monocyte product, similar to interleukin 1 (IL 1), is a powerful stimulator of bone resorption in vitro. However, the possibility remained that other immune cell products may contribute to OAF activity. We have therefore tested three recombinant cytokines; IL 1, interleukin 2 (IL 2), and interferon-gamma (IFN-gamma) for their activity in a neonatal mouse bone resorption assay. We report here that purified recombinant murine IL 1 is a potent and powerful stimulator of bone resorption in vitro, active over a concentration range of 0.14 to 33 U/ml (1.3 X 10(-12) to 3.1 X 10(-10) M). IL 1-stimulated bone resorption was unaffected by cyclooxygenase inhibition but was inhibited by calcitonin and IFN-gamma. IL 2 had no effect on bone resorption.
人外周血细胞在体外培养时会释放骨吸收因子，这些因子被称为破骨细胞激活因子（OAF），但仍未被鉴定出来。我们之前表明，一种类似于白细胞介素1（IL-1）的单核细胞产物是体外骨吸收的强大刺激物。然而，其他免疫细胞产物可能促成OAF活性的可能性仍然存在。因此，我们在新生小鼠骨吸收试验中测试了三种重组细胞因子；IL-1、白细胞介素2（IL-2）和干扰素-γ（IFN-γ）的活性。我们在此报告，纯化的重组小鼠IL-1是体外骨吸收的有效且强大的刺激物，在0.14至33 U/ml（1.3×10⁻¹²至3.1×10⁻¹⁰ M）的浓度范围内具有活性。IL-1刺激的骨吸收不受环氧化酶抑制的影响，但受降钙素和IFN-γ的抑制。IL-2对骨吸收没有影响。
