Department of Biotechnology, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.
J Cell Biochem. 2019 Aug;120(8):12422-12428. doi: 10.1002/jcb.28508. Epub 2019 Feb 28.
Gastrointestinal (GI) microflora is an important system in the host, as it has both pathogenic and probiotic bacteria. Most of the studies were focused on the human gut microflora and the available information on the intestinal microflora of goats was limited. This urged the need to inspect the impacts of the goat's gut microflora. Metagenomic investigation of probiotic bacteria in the GI tract of goat is one of the challenging streams because of the less available data of the uncultivable bacteria. In our report, comparative analysis of metagenomic and enrichment samples of goat intestinal content was done and this approach will be helpful in analyzing the identification of uncultivable and cultivable probiotic bacteria. This study mainly focused on three key probiotic adhesion genes, such as EF-Tu, mapA, and mub. The GI of four different goats were investigated for these genes. The data from this study showed that there is a wide diversity of these genes among goat intestinal samples.
胃肠道（GI）微生物群是宿主的一个重要系统，因为它既有致病细菌，也有有益细菌。大多数研究都集中在人类肠道微生物群上，而关于山羊肠道微生物群的可用信息有限。这促使人们需要检查山羊肠道微生物群的影响。由于无法培养的细菌的数据较少，因此对胃肠道中益生菌的宏基因组学研究是具有挑战性的领域之一。在我们的报告中，对山羊肠道内容物的宏基因组和富集样本进行了比较分析，这种方法将有助于分析无法培养和可培养益生菌的鉴定。这项研究主要集中在三个关键的益生菌黏附基因，如 EF-Tu、mapA 和 mub 上。对四只不同山羊的胃肠道进行了这些基因的研究。这项研究的数据表明，在山羊肠道样本中，这些基因存在广泛的多样性。
