Kavand Hanie, Rahaie Mahdi, Koohsorkhi Javad, Haghighipour Nooshin, Bonakdar Shahin
Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
Advanced Micro and Nano Devices Lab, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
Biotechnol Appl Biochem. 2019 May;66(3):445-453. doi: 10.1002/bab.1741. Epub 2019 Apr 1.
Cell function regulation is influenced by continuous biochemical and biophysical signal exchange within the body. Substrates with nano/micro-scaled topographies that mimic the physiological niche are widely applied for tissue engineering applications. As the cartilage niche is composed of several stimulating factors, a multifunctional substrate providing topographical features while having the capability of electrical stimulation is presented. Herein, we demonstrate a biocompatible and conductive chondrocyte cell-imprinted substrate using polydimethylsiloxane (PDMS) and carbon nanotubes (CNTs) as conductive fillers. Unlike the conventional silicon wafers or structural photoresist masters used for molding, cell surface topographical replication is challenging as biological cells showed extremely sensitive to chemical solvent residues during molding. The composite showed no significant difference compared with PDMS with regard to cytotoxicity, whereas an enhanced cell adhesion was observed on the conductive composite's surface. Integration of nanomaterials into the cell seeding scaffolds can make tissue regeneration process more efficient.
细胞功能调节受体内持续的生化和生物物理信号交换影响。具有模拟生理微环境的纳米/微米级形貌的基质被广泛应用于组织工程领域。由于软骨微环境由多种刺激因子组成，因此提出了一种具有形貌特征且具备电刺激能力的多功能基质。在此，我们展示了一种使用聚二甲基硅氧烷（PDMS）和碳纳米管（CNTs）作为导电填料的生物相容性和导电性软骨细胞印记基质。与用于成型的传统硅片或结构光刻胶母版不同，细胞表面形貌复制具有挑战性，因为生物细胞在成型过程中对化学溶剂残留极其敏感。该复合材料在细胞毒性方面与PDMS相比无显著差异，而在导电复合材料表面观察到细胞粘附增强。将纳米材料整合到细胞接种支架中可以使组织再生过程更高效。
