State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, People's Republic of China.
Biomed Mater. 2017 Jul 4;12(4):045004. doi: 10.1088/1748-605X/aa6e27.
A major issue in bone tissue engineering is the selection of biocompatible materials for implants, to reduce unwanted inflammatory reactions and promote cell adhesion. Bone tissue growth on suitable biomedical implants can shorten recovery and hospitalization after surgery. Therefore, a method to improve tissue-implant integration and healing would be of scientific and clinical interest. In this work, we permeated polydimethylsiloxane (PDMS) into carbon/carbon (C/C) composites (PDMS-C/C) and then coated it with 4,5-dihydroxyanthraquinone-2-carboxylic acid (rhein) to create rhein-PDMS-C/C to increase its biocompatibility and reduce the occurrence of inflammatory reactions. We measured in vitro adhesion and proliferation of MC3T3-E1 cells and bacteria to evaluate the biocompatibility and antimicrobial properties of C/C, PDMS-C/C, and rhein-PDMS-C/C. In vivo, x-ray and micro-CT evaluation three, six and nine weeks after surgery revealed that rhein-PDMS-C/C was more effective than PDMS-C/C and C/C composite in terms of antibacterial activity, cell adhesion and tissue growth. Compared with C/C and PDMS-C/C, rhein-PDMS-C/C could be suitable for clinical applications for bone tissue engineering.
在骨组织工程中，一个主要问题是选择生物相容性材料用于植入物，以减少不必要的炎症反应并促进细胞黏附。合适的生物医学植入物上的骨组织生长可以缩短手术后的恢复和住院时间。因此，改善组织-植入物整合和愈合的方法将具有科学和临床意义。在这项工作中，我们将聚二甲基硅氧烷（PDMS）渗透到碳/碳（C/C）复合材料（PDMS-C/C）中，然后用 4,5-二羟基蒽醌-2-羧酸（大黄酸）对其进行涂层，以创建大黄酸-PDMS-C/C，从而提高其生物相容性并减少炎症反应的发生。我们测量了 MC3T3-E1 细胞和细菌在体外的黏附和增殖情况，以评估 C/C、PDMS-C/C 和大黄酸-PDMS-C/C 的生物相容性和抗菌性能。体内实验在手术后三、六和九周进行 X 射线和 micro-CT 评估，结果表明，在抗菌活性、细胞黏附和组织生长方面，大黄酸-PDMS-C/C 比 PDMS-C/C 和 C/C 复合材料更有效。与 C/C 和 PDMS-C/C 相比，大黄酸-PDMS-C/C 可能适用于骨组织工程的临床应用。
