Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; The Graduate School, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Chiang Mai University, Chang Mai 50200, Thailand; Research Center on Chemistry for Development of Health Promoting Products from Northern Resources, Chiang Mai University, Chiang Mai 50200, Thailand.
Bioelectrochemistry. 2019 Jun;127:125-135. doi: 10.1016/j.bioelechem.2019.01.008. Epub 2019 Feb 13.
A facile one-step electrochemical synthesis of a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) (Pt/rGO/P3ABA) nanocomposite film on a screen-printed carbon electrode (SPCE) and its application in the development of sensitive amperometric biosensors was successfully demonstrated herein. The electropolymerization of P3ABA together with co-electrodeposition of rGO and Pt was conducted by cyclic voltammetry, as was the GO reduction to rGO. A Pt/rGO/P3ABA-modified SPCE exhibited excellent electrocatalytic oxidation towards hydrogen peroxide (HO) and can be employed as an electrochemical platform for the immobilization of glucose oxidase (GOx) and cholesterol oxidase (ChOx) to fabricate glucose and cholesterol biosensors, respectively. Under the optimized conditions at a working potential of +0.50 V, the proposed biosensors revealed excellent linear responses to glucose and cholesterol in the concentration ranges of 0.25-6.00 mM and 0.25-4.00 mM, respectively, with high sensitivities of 22.01 and 15.94 μA mM cm and low detection limits (LODs) of 44.3 and 40.5 μM. Additionally, the Michaelis-Menten constant (K) of GOx was 3.54 mM, while the K of ChOx was 3.82 mM. Both biosensors displayed a good anti-interference ability and clearly exhibited acceptable recoveries for the detection of glucose and cholesterol in a human serum sample (98.2-104.1%).
本文成功展示了一种简便的电化学合成方法，可在丝网印刷碳电极（SPCE）上一步合成铂/还原氧化石墨烯/聚（3-氨基苯甲酸）（Pt/rGO/P3ABA）纳米复合膜，并将其应用于灵敏安培生物传感器的开发。通过循环伏安法进行了 P3ABA 的电聚合以及 rGO 和 Pt 的共电沉积，同时也实现了 GO 向 rGO 的还原。Pt/rGO/P3ABA 修饰的 SPCE 对过氧化氢（HO）表现出优异的电催化氧化性能，可作为固定葡萄糖氧化酶（GOx）和胆固醇氧化酶（ChOx）的电化学平台，分别用于制备葡萄糖和胆固醇生物传感器。在工作电位为+0.50 V 的优化条件下，所提出的生物传感器对葡萄糖和胆固醇的线性响应范围分别为 0.25-6.00 mM 和 0.25-4.00 mM，灵敏度分别为 22.01 和 15.94 μA mM cm 和低检测限（LOD）分别为 44.3 和 40.5 μM。此外，GOx 的米氏常数（K）为 3.54 mM，而 ChOx 的 K 为 3.82 mM。两种生物传感器均表现出良好的抗干扰能力，并且在人血清样品中检测葡萄糖和胆固醇时显示出可接受的回收率（98.2-104.1%）。
