两种快固型盖髓材料对人牙髓干细胞活力和成骨分化影响的体外研究。
Effects of two fast-setting pulp-capping materials on cell viability and osteogenic differentiation in human dental pulp stem cells: An in vitro study.
机构信息
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Conservative Dentistry and Endodontics, West China School and Hospital of Stomatology, Sichuan University, Chengdu, 610041, China; Department of Conservative Dentistry and Endodontics, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, China.
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China School and Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
出版信息
Arch Oral Biol. 2019 Apr;100:100-105. doi: 10.1016/j.archoralbio.2019.02.014. Epub 2019 Feb 20.
OBJECTIVE
The purpose of this study was to compare the effects of two fast-setting pulp-capping materials, Biodentine (BD) and iRoot Fast Set (FS) root repair material, on the attachment, viability, migration, and differentiation of human dental pulp stem cells (hDPSCs).
METHODS
A comparative study was conducted between BD and FS material disks. Scanning electron microscope (SEM) images were used to observe the attachment of hDPSCs on the disks. A live/dead assay was used to assess the cell viability. Transwell assay was performed to study cell migration. Cell differentiation was determined by quantitative real-time polymerase chain reaction (qRT-PCR) for the analysis of osteogenic differentiation gene expression: alkaline phosphatase (ALP), collagen type I (COL1) and osteocalcin (OCN).
RESULTS
SEM images indicated that hDPSCs showed a well-spreading morphology on both BD and FS disks. FS significantly increased the proliferation and migration of hDPSCs on day 7 (P＜0.05). Neither BD nor FS promoted the expression of osteogenic genes during the observation period.
CONCLUSIONS
BD and FS both were beneficial to hDPSC attachment, and they had similar effects on cell osteogenic differentiation, whereas FS performed better than BD on hDPSCs proliferation and migration.
目的
本研究旨在比较两种快固型盖髓材料，即 Biodentine（BD）和 iRoot Fast Set（FS）根充修复材料，对人牙髓干细胞（hDPSCs）黏附、活力、迁移和分化的影响。
方法
对 BD 和 FS 材料盘进行了比较研究。扫描电子显微镜（SEM）图像用于观察 hDPSCs 在盘上的黏附情况。使用活/死检测评估细胞活力。Transwell 测定用于研究细胞迁移。通过定量实时聚合酶链反应（qRT-PCR）分析成骨分化基因表达：碱性磷酸酶（ALP）、I 型胶原（COL1）和骨钙素（OCN），以确定细胞分化。
结果
SEM 图像表明，hDPSCs 在 BD 和 FS 盘上均呈现良好的扩展形态。FS 在第 7 天显著增加了 hDPSCs 的增殖和迁移（P＜0.05）。在观察期间，BD 和 FS 均未促进成骨基因的表达。
结论
BD 和 FS 均有利于 hDPSC 黏附，对细胞成骨分化的影响相似，而 FS 在 hDPSCs 增殖和迁移方面的表现优于 BD。
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