一项实验室研究，旨在测试三种牙髓盖髓生物材料提取物对人牙髓干细胞反应的影响。

A laboratory study to test the responses of human dental pulp stem cells to extracts from three dental pulp capping biomaterials.

机构信息

Department of Endodontics, Xiangya School of Stomatology, Central South University, Changsha, China.

Department of Operative Dentistry, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.

出版信息

Int Endod J. 2021 Jul;54(7):1118-1128. doi: 10.1111/iej.13495. Epub 2021 Mar 7.

DOI:10.1111/iej.13495

PMID:33567103

Abstract

AIM

This laboratory study aimed to investigate the effects of three endodontic biomaterials; MTA-HP, iRoot-BP-Plus and ACTIVA on the proliferation, adhesion and osteogenic differentiation of human Dental Pulp Stem Cells (hDPSCs).

METHODOLOGY

The hDPSCs were isolated from the dental pulps of 21 patients scheduled for surgical extraction of their impacted third molars. The MTT assay was used for assessing cellular proliferation. Ninety-six-well plates were used and the experiment was repeated four times under the same condition and the assay was done in triplicate. Four groups were assigned in which the hDPSCs were cultured in complete media only and considered as negative control. Whilst in the 2 , 3 and 4 groups, the cells were treated with CM supplemented with 1.5 μl MTA-HP (CM-MTA, iRoot-BP-Plus (CM-BP), and ACTIVA(CM-AC) extracts, respectively. Attachment adhesion and growth morphology of hDPSCs were observed using SEM and the osteogenic differentiation assay was evaluated by Alizarin red stain test (ARS). The data of proliferation and osteogenic differentiation were analysed using two-way ANOVA followed by Tukey's post hoc multiple comparison test. A p-value < 0.05 was considered significant to analyse the differences amongst the means of groups.

RESULTS

Both CM-MTA and CM-BP groups were associated with a significant increase in hDPSC proliferation in comparison with CM-AC and CM groups (p = 0.001). hDPSCs exhibited a greater cellular attachment to iRoot-BP-Plus surfaces followed by MTA-HP, whilst less attachment was observed in the ACTIVA group. Moreover, at day 7 there was a significant difference in formation of mineralizing nodules; CM-BP, CM-MTA and CM-AC groups respectively (p = 0.001). Whilst there was no significance of difference between CM-AC and CM groups (p > 0.05).

CONCLUSIONS

In a laboratory setting, ACTIVA, MTA-HP and iRoot-BP-Plus promoted hDPSCs proliferation, mineralization and attachment, which may explain their in-situ success as endodontic biomaterials.

摘要

目的

本实验室研究旨在探讨三种根管生物材料 MTA-HP、iRoot-BP-Plus 和 ACTIVA 对人牙髓干细胞（hDPSCs）增殖、黏附和成骨分化的影响。

方法

从 21 名因第三磨牙阻生需行手术拔除的患者牙髓中分离 hDPSCs。采用 MTT 法检测细胞增殖。使用 96 孔板，在相同条件下重复实验 4 次，一式三份进行检测。将 hDPSCs 分别培养在完全培养基（阴性对照）和补充 1.5 μl MTA-HP（CM-MTA）、iRoot-BP-Plus（CM-BP）和 ACTIVA（CM-AC）提取物的培养基中，共分为 4 组。采用 SEM 观察 hDPSCs 的黏附、生长形态，采用茜素红染色试验（ARS）评价成骨分化试验。采用双因素方差分析（ANOVA）和 Tukey 事后多重比较检验分析增殖和成骨分化数据。组间均值比较采用 p<0.05 表示差异有统计学意义。

结果

与 CM-AC 和 CM 组相比，CM-MTA 和 CM-BP 组 hDPSC 增殖显著增加（p=0.001）。hDPSCs 与 iRoot-BP-Plus 表面的黏附力更强，其次是 MTA-HP，而与 ACTIVA 组的黏附力较弱。此外，第 7 天时，CM-BP、CM-MTA 和 CM-AC 组矿化结节形成有显著差异（p=0.001）。而 CM-AC 和 CM 组之间无显著性差异（p>0.05）。