Sun Yan, Luo Tao, Shen Ya, Haapasalo Markus, Zou Ling, Liu Jun
State Key Laboratory of Oral Diseases, Department of Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia, Canada.
PLoS One. 2017 Oct 23;12(10):e0186848. doi: 10.1371/journal.pone.0186848. eCollection 2017.
The present study investigated the effect of iRoot Fast Set root repair material (iRoot FS) on the proliferation, migration and differentiation of human dental pulp stem cells (hDPSCs). The hDPSCs were treated with eluates of iRoot FS at concentrations of 0.2 and 2 mg/mL, referred to as FS0.2 and FS2, respectively, and Biodentine (BD; Septodont, Saint Maur des Faussés, France) eluates at the corresponding concentrations as positive controls. A CCK8 assay was performed to determine cell proliferation. Wound healing and transwell assays were conducted to examine cell migration. Osteogenic differentiation was evaluated based on alkaline phosphatase activity, Alizarin Red S staining and quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) to analyze the mRNA expression of differentiation gene markers. Cell proliferation was higher in the FS and BD groups than in the blank controls at 3 and 7 days. Moreover, FS0.2 enhanced cell migration and significantly promoted the osteogenic differentiation of hDPSCs. These findings suggested that iRoot FS is a bioactive material that promotes the proliferation, migration and osteogenic differentiation of hDPSCs.
本研究调查了iRoot Fast Set根修复材料（iRoot FS）对人牙髓干细胞（hDPSCs）增殖、迁移和分化的影响。将hDPSCs分别用浓度为0.2和2 mg/mL的iRoot FS洗脱液处理，分别称为FS0.2和FS2，并将相应浓度的碧兰麻（BD；法国圣莫尔代福塞Septodont公司）洗脱液作为阳性对照。进行CCK8测定以确定细胞增殖。进行伤口愈合和transwell测定以检查细胞迁移。基于碱性磷酸酶活性、茜素红S染色和定量实时逆转录聚合酶链反应（qRT-PCR）评估成骨分化，以分析分化基因标志物的mRNA表达。在第3天和第7天，FS组和BD组的细胞增殖高于空白对照组。此外，FS0.2增强了细胞迁移并显著促进了hDPSCs的成骨分化。这些发现表明，iRoot FS是一种促进hDPSCs增殖、迁移和成骨分化的生物活性材料。
