Jung H W, Yun W S, Hahm Y I, Kim K-H
School of Agricultural Biotechnology and Research Center for New Biomaterials in Agriculture, Seoul National University, Suwon 441-744, Korea.
Alpine Agriculture Experiment Station, Pyungchang, Kangwon 232-955, Korea.
Plant Dis. 2002 Feb;86(2):112-117. doi: 10.1094/PDIS.2002.86.2.112.
Four isolates of Tobacco mosaic virus (TMV-potato 1 to 4) were obtained from potato plants (Solanum tuberosum) in cultivated potato plantings in Korea. These isolates were differentiated based on biological properties, symptomatology, and nucleotide sequence analysis of the coat protein (CP) gene. TMV potato isolates caused typical symptoms on 20 inoculated plant species as compared to the type (U1) TMV strain. The four isolates each produced distinctly different symptoms on Gomphrena globosa. In contrast to the type strain of TMV, infections with two of the isolates reported here were not restricted to inoculated leaves of G. globosa but moved systemically through the plants. In addition, three additional systemic hosts (Chenopodium amaranticolor, C. quinoa, and C. murale) for TMV were revealed. Sequence analysis of the CP gene differentiated TMV-potato isolates. The CP gene sequence exhibited significant identity (83.1 to 99.2%) among TMV-potato isolates while showing 88.1 to 99.4% identities on predicted amino acid sequences. Based on a comparison of the CP gene nucleotide and deduced amino acid sequences between TMV-potato isolates and other TMV strains, TMV-potato 1, 3, and 4 were closely related to TMV strains U1, U2, V-FAVA, and NC82 with 98.8 to 100% identity. In contrast, TMV-potato 2 was closely related to TMV strains L, KP, KO-TOB, K1, and K2 with 93.8 to 98.8% identity.
从韩国栽培马铃薯种植区的马铃薯植株（茄属马铃薯）中获得了4株烟草花叶病毒分离株（TMV-马铃薯1至4）。这些分离株基于生物学特性、症状学以及外壳蛋白（CP）基因的核苷酸序列分析进行区分。与典型（U1）TMV株系相比，TMV马铃薯分离株在20种接种植物物种上引起典型症状。这4个分离株在千日红上各自产生明显不同的症状。与TMV的典型株系不同，这里报道的两个分离株的感染不限于千日红的接种叶片，而是在植株内系统传播。此外，还发现了TMV的另外3种系统寄主（苋色藜、藜麦和墙生藜）。CP基因的序列分析区分了TMV马铃薯分离株。CP基因序列在TMV马铃薯分离株之间显示出显著的一致性（83.1%至99.2%），而预测的氨基酸序列一致性为88.1%至99.4%。基于TMV马铃薯分离株与其他TMV株系之间CP基因核苷酸和推导氨基酸序列的比较，TMV-马铃薯1、3和4与TMV株系U1、U2、V-FAVA和NC82密切相关，一致性为98.8%至100%。相比之下，TMV-马铃薯2与TMV株系L、KP、KO-TOB、K1和K2密切相关，一致性为93.8%至98.8%。
