Kanwal Zakia, Raza Muhammad Akram, Manzoor Farkhanda, Riaz Saira, Jabeen Ghazala, Fatima Shafaq, Naseem Shahzad
Department of Zoology, Faculty of Natural Science, Lahore College for Women University, Jail Road Lahore 54000, Pakistan.
Centre of Excellence in Solid State Physics, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan.
Nanomaterials (Basel). 2019 Feb 25;9(2):309. doi: 10.3390/nano9020309.
In the present in vivo study, we provide a comparison of toxicological consequences induced by four different types of spherical nanoparticles (NPs)-silver nanoparticles (AgNPs, 40 ± 6 nm), nickel (NiNPs, 43 ± 6 nm), cobalt oxide (Co₃O₄NPs, 60 ± 6 nm), and chromium oxide (Cr₃O₄NPs, 50 ± 5 nm)-on freshwater fish . Fish were exposed to NPs (25 mg/L) for 21 days. We observed a NPs type-dependent toxicity in fish. An altered behavior showing signs of stress and a substantial reduction in total leukocyte count was noticed in all NP-treated groups. A low total erythrocyte count in all NP-treated fish except for Co₃O₄NPs was discerned while a low survival rate in the case of Cr₃O₄NP-treated fish was observed. A significant decrease in growth and hemoglobin were noticed in NiNP- and Cr₃O₄NP-treated fish. A considerable total protein elevation was detected in NiNP-, Co₃O₄NP-, and Cr₃O₄NP-treated groups. An upgrading in albumin level was witnessed in Co₃O₄NP- and Cr₃O₄NP-treated groups while a high level of globulin was noted in NiNP- and Co₃O₄NP-exposed groups. In all NP-treated groups, a depleted activity of antioxidative enzymes and pathological lesions in liver and kidney were noticed.
在本体内研究中，我们比较了四种不同类型的球形纳米颗粒（NPs）——银纳米颗粒（AgNPs，40±6纳米）、镍纳米颗粒（NiNPs，43±6纳米）、氧化钴（Co₃O₄NPs，60±6纳米）和氧化铬（Cr₃O₄NPs，50±5纳米）——对淡水鱼的毒理学影响。将鱼暴露于纳米颗粒（25毫克/升）中21天。我们观察到鱼体内纳米颗粒类型依赖性毒性。在所有纳米颗粒处理组中均观察到行为改变，表现出应激迹象，总白细胞计数大幅减少。除Co₃O₄NPs外，在所有纳米颗粒处理的鱼中均发现总红细胞计数较低，而在Cr₃O₄NP处理的鱼中观察到低存活率。在NiNP和Cr₃O₄NP处理的鱼中，生长和血红蛋白显著下降。在NiNP、Co₃O₄NP和Cr₃O₄NP处理组中检测到总蛋白显著升高。在Co₃O₄NP和Cr₃O₄NP处理组中观察到白蛋白水平升高，而在NiNP和Co₃O₄NP暴露组中观察到球蛋白水平较高。在所有纳米颗粒处理组中，均观察到抗氧化酶活性降低以及肝脏和肾脏出现病理损伤。
