人戊二醛固定红细胞的表面电荷
The surface charge of human glutaraldehyde-fixed erythrocytes.
作者信息
Grunze M, Heuck C C
出版信息
Biochim Biophys Acta. 1986 Mar 27;856(1):41-4. doi: 10.1016/0005-2736(86)90007-6.
We measured the number of charged residues at the surface of fresh human erythrocytes after fixation with glutaraldehyde by polyelectrolyte titration using polycations of different chemical composition and various molecular weights. Independent of the reagents used, the number was (8.5 +/- 1.5) X 10(8) negatively charged residues per cell. The surface charge density of 6.3 e/nm2 is considerably higher than that calculated from the electrophoretic mobility for which the surface charge density is calculated to be 0.09 e/nm2.
我们通过使用不同化学组成和各种分子量的聚阳离子进行聚电解质滴定，测量了经戊二醛固定后的新鲜人红细胞表面带电残基的数量。无论使用何种试剂，每个细胞的数量为(8.5±1.5)×10⁸个带负电残基。表面电荷密度为6.3 e/nm²，远高于根据电泳迁移率计算得出的表面电荷密度，后者经计算为0.09 e/nm²。
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