Institute of Special Animals and Plants Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China.
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
Molecules. 2019 Mar 1;24(5):870. doi: 10.3390/molecules24050870.
Suppressive effects of ginsenoside Rh₂ (Rh₂), (24)-pseudo-ginsenoside HQ (-PHQ), and (24)-pseudo-ginsenoside HQ (-PHQ) against lipopolysaccharide (LPS)-induced depression-like behavior were evaluated using the forced swimming test (FST) and tail suspension test (TST) in mice. Pretreatment with Rh₂, -PHQ, and -PHQ significantly decreased immobility time in FST and TST with clear dose-dependence, and significantly downregulated levels of serum tumor necrosis factor-α and interleukin-6, and upregulated superoxide dismutase activity in the hippocampus of LPS-challenged mice. Furthermore, -PHQ and -PHQ significantly increased the expression of the brain-derived neurotrophic factor (BDNF), tropomyosin-related kinase B (TrkB), sirtuin type 1 (Sirt1), and nuclear-related factor 2, and inhibited the phosphorylation of inhibitor of κB-α and nuclear factor-κB (NF-κB) in the hippocampus of LPS-challenged mice. Additionally, the antidepressant-like effect of -PHQ was found related to the dopaminergic (DA), γ-aminobutyric acid (GABA)ergic, and noradrenaline systems, while the antidepressive effect of -PHQ was involved in the DA and GABAergic systems. Taken together, these results suggested that Rh₂, -PHQ, and -PHQ produced significant antidepressant-like effects, which may be related to the BDNF/TrkB and Sirt1/NF-κB signaling pathways.
用小鼠强迫游泳试验（FST）和悬尾试验（TST）评价了人参皂苷 Rh₂（Rh₂）、（24）-伪人参皂苷 HQ（-PHQ）和（24）-伪人参皂苷 HQ（-PHQ）对脂多糖（LPS）诱导的抑郁样行为的抑制作用。Rh₂、-PHQ 和 -PHQ 预处理可显著降低 LPS 应激小鼠 FST 和 TST 的不动时间，呈明显的剂量依赖性，并显著下调血清肿瘤坏死因子-α和白细胞介素-6水平，上调超氧化物歧化酶活性海马区。此外，-PHQ 和 -PHQ 显著增加脑源性神经营养因子（BDNF）、原肌球蛋白相关激酶 B（TrkB）、沉默调节蛋白 1（Sirt1）和核相关因子 2 的表达，并抑制磷酸化抑制剂κB-α和核因子-κB（NF-κB）在 LPS 应激小鼠的海马区。此外，-PHQ 的抗抑郁作用与多巴胺（DA）、γ-氨基丁酸（GABA）能和去甲肾上腺素能系统有关，而 -PHQ 的抗抑郁作用与 DA 和 GABA 能系统有关。综上所述，这些结果表明 Rh₂、-PHQ 和 -PHQ 产生了显著的抗抑郁样作用，这可能与 BDNF/TrkB 和 Sirt1/NF-κB 信号通路有关。
