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随意编辑:先进基因组学和基因组编辑时代的基因型独立植物转化。

Edit at will: Genotype independent plant transformation in the era of advanced genomics and genome editing.

机构信息

Department of Cell and Molecular Biology, University of Rhode Island, RI 02892, USA.

Department of Cell and Molecular Biology, University of Rhode Island, RI 02892, USA.

出版信息

Plant Sci. 2019 Apr;281:186-205. doi: 10.1016/j.plantsci.2019.01.006. Epub 2019 Jan 14.

Abstract

The combination of advanced genomics, genome editing and plant transformation biology presents a powerful platform for basic plant research and crop improvement. Together these advances provide the tools to identify genes as targets for direct editing as single base pair changes, deletions, insertions and site specific homologous recombination. Recent breakthrough technologies using morphogenic regulators in plant transformation creates the ability to introduce reagents specific toward their identified targets and recover stably transformed and/or edited plants which are genotype independent. These technologies enable the possibility to alter a trait in any variety, without genetic disruption which would require subsequent extensive breeding, but rather to deliver the same variety with one trait changed. Regulatory issues regarding this technology will predicate how broadly these technologies will be implemented. In addition, education will play a crucial role for positive public acceptance. Taken together these technologies comprise a platform for advanced breeding which is an imperative for future world food security.

摘要

先进的基因组学、基因组编辑和植物转化生物学的结合为基础植物研究和作物改良提供了一个强大的平台。这些进展共同提供了工具,可以将基因作为直接编辑的目标,进行单碱基对变化、缺失、插入和特定位点同源重组。最近使用植物转化中的形态发生调节剂的突破性技术创造了引入针对其已识别目标的试剂的能力,并能稳定转化和/或编辑植物,而与基因型无关。这些技术使得改变任何品种的特性成为可能,而无需遗传破坏,这将需要随后进行广泛的繁殖,而只是改变一个特性。关于这项技术的监管问题将决定这项技术的广泛实施程度。此外,教育将在公众的积极接受方面发挥关键作用。这些技术共同构成了一个高级育种平台,这是未来世界粮食安全的当务之急。

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