采用酶免疫测定法对肺炎支原体感染进行血清学诊断。
Serological diagnosis of Mycoplasma pneumoniae infection by enzyme immunoassay.
作者信息
Räisänen S M, Suni J I, Leinikki P
出版信息
J Clin Pathol. 1980 Sep;33(9):836-40. doi: 10.1136/jcp.33.9.836.
Abstract
Antibodies against Mycoplasma pneumoniae antigen obtained by Tween-ether treatment from purified M. pneumoniae were measured by means of enzyme-linked immunosorbent assay (ELISA). Paired sera from 19 patients with pneumonia and from 13 patients with acute pancreatitis with a significant rise in complement fixing antibodies against M. pneumoniae were studied. Single sera from healthy 1-year-old children were used as controls. High levels of IgG and IgM class antibodies were seen in sera from patients with pneumonia while most patients with acute pancreatitis and all the children showed low levels of antibodies. The results indicate that ELISA using Tween-ether treated M. pneumoniae antigen could be used successfully in the specific laboratory diagnosis of M. pneumoniae infection.
摘要
采用酶联免疫吸附测定法（ELISA）检测了经吐温 - 乙醚处理从纯化的肺炎支原体获得的抗肺炎支原体抗原的抗体。研究了19例肺炎患者和13例急性胰腺炎患者的配对血清，这些患者针对肺炎支原体的补体结合抗体显著升高。将1岁健康儿童的单份血清用作对照。肺炎患者血清中可见高水平的IgG和IgM类抗体，而大多数急性胰腺炎患者和所有儿童的抗体水平较低。结果表明，使用经吐温 - 乙醚处理的肺炎支原体抗原的ELISA可成功用于肺炎支原体感染的特异性实验室诊断。
相似文献
1
Serological diagnosis of Mycoplasma pneumoniae infection by enzyme immunoassay.采用酶免疫测定法对肺炎支原体感染进行血清学诊断。
J Clin Pathol. 1980 Sep;33(9):836-40. doi: 10.1136/jcp.33.9.836.
2
Measurement of IgM antibodies in the diagnosis of mycoplasma pneumonia.IgM抗体检测在支原体肺炎诊断中的应用
Pathology. 1980 Oct;12(4):519-24. doi: 10.3109/00313028009086804.
3
Development of urease conjugated enzyme-linked immunosorbent assays (ELISA) for the detection of IgM and IgG antibodies against Mycoplasma pneumoniae in human sera.用于检测人血清中抗肺炎支原体IgM和IgG抗体的脲酶偶联酶联免疫吸附测定（ELISA）的开发。
Diagn Microbiol Infect Dis. 1988 Oct;11(2):101-7. doi: 10.1016/0732-8893(88)90078-8.
4
Evaluation of a latex agglutination test for detection of immunoglobulin M (IgM) and IgG antibodies to Mycoplasma pneumoniae.用于检测肺炎支原体免疫球蛋白M（IgM）和免疫球蛋白G（IgG）抗体的乳胶凝集试验评估
Eur J Clin Microbiol Infect Dis. 2002 May;21(5):417-8. doi: 10.1007/s10096-002-0721-5. Epub 2002 May 7.
5
Use of the enzyme-linked immunosorbent assay for the early diagnosis of Mycoplasma pneumoniae infection.酶联免疫吸附测定法在肺炎支原体感染早期诊断中的应用。
Eur J Clin Microbiol. 1984 Apr;3(2):116-21. doi: 10.1007/BF02014328.
6
Reaction pattern of human anti-Mycoplasma pneumoniae antibodies in enzyme-linked immunosorbent assays and immunoblotting.人抗肺炎支原体抗体在酶联免疫吸附测定和免疫印迹中的反应模式。
J Clin Microbiol. 1986 Mar;23(3):517-22. doi: 10.1128/jcm.23.3.517-522.1986.
7
Serodiagnosis of M. pneumoniae infections by enzyme-linked immunosorbent assay (ELISA).通过酶联免疫吸附测定（ELISA）对肺炎支原体感染进行血清学诊断。
Yale J Biol Med. 1983 Sep-Dec;56(5-6):517-21.
8
The serological diagnosis of Mycoplasma pneumoniae infection: a comparison of complement fixation, haemagglutination and immunofluorescence.肺炎支原体感染的血清学诊断：补体结合试验、血凝试验及免疫荧光法的比较
J Hyg (Lond). 1985 Oct;95(2):345-52. doi: 10.1017/s0022172400062768.
9
Determination of antibody to Mycoplasma pneumoniae by enzyme-linked immunosorbent assay II. Serum antibody in patients with Mycoplasma pneumoniae infections.用酶联免疫吸附测定法测定肺炎支原体抗体 II. 肺炎支原体感染患者的血清抗体
Sci Rep Res Inst Tohoku Univ Med. 1984 Oct;31(1-4):13-26.
10
Comparison of enzyme-linked immunosorbent assay (ELISA) and complement fixation test for detection of Mycoplasma pneumoniae antibodies.酶联免疫吸附测定（ELISA）与补体结合试验检测肺炎支原体抗体的比较。
J Clin Pathol. 1983 Feb;36(2):228-32. doi: 10.1136/jcp.36.2.228.
引用本文的文献
1
Identification, expression and serological evaluation of the recombinant ATP synthase beta subunit of Mycoplasma pneumoniae.肺炎支原体 ATP 合酶β亚单位的鉴定、表达及血清学评估。
BMC Microbiol. 2010 Aug 11;10:216. doi: 10.1186/1471-2180-10-216.
2
Evaluation of four commercial immunoglobulin G (IgG)- and IgM-specific enzyme immunoassays for diagnosis of Mycoplasma pneumoniae infections.评估四种用于诊断肺炎支原体感染的商业免疫球蛋白G（IgG）和免疫球蛋白M（IgM）特异性酶免疫测定法。
J Clin Microbiol. 2002 Jan;40(1):165-71. doi: 10.1128/JCM.40.1.165-171.2002.
3
Diagnosis of Mycoplasma pneumoniae pneumonia in children.儿童支原体肺炎的诊断
J Clin Microbiol. 1998 Nov;36(11):3155-9. doi: 10.1128/JCM.36.11.3155-3159.1998.
4
Measurement of antibody to Ureaplasma urealyticum by an enzyme-linked immunosorbent assay and detection of antibody responses in patients with nongonococcal urethritis.通过酶联免疫吸附测定法检测解脲脲原体抗体及非淋菌性尿道炎患者抗体反应的检测
J Clin Microbiol. 1983 Feb;17(2):288-95. doi: 10.1128/jcm.17.2.288-295.1983.
5
Comparison of enzyme-linked immunosorbent assay (ELISA) and complement fixation test for detection of Mycoplasma pneumoniae antibodies.酶联免疫吸附测定（ELISA）与补体结合试验检测肺炎支原体抗体的比较。
J Clin Pathol. 1983 Feb;36(2):228-32. doi: 10.1136/jcp.36.2.228.
6
False-positive complement fixing antibodies against Mycoplasma pneumoniae in patients with bacterial meningitis.细菌性脑膜炎患者中针对肺炎支原体的假阳性补体结合抗体
J Clin Pathol. 1982 Aug;35(8):903. doi: 10.1136/jcp.35.8.903.
7
Mycoplasma pneumoniae protein involved in the antibody response in human infection.肺炎支原体蛋白参与人类感染中的抗体反应。
J Clin Pathol. 1984 Oct;37(10):1129-33. doi: 10.1136/jcp.37.10.1129.
8
Serodiagnosis of M. pneumoniae infections by enzyme-linked immunosorbent assay (ELISA).通过酶联免疫吸附测定（ELISA）对肺炎支原体感染进行血清学诊断。
Yale J Biol Med. 1983 Sep-Dec;56(5-6):517-21.
9
Use of the enzyme-linked immunosorbent assay for the early diagnosis of Mycoplasma pneumoniae infection.酶联免疫吸附测定法在肺炎支原体感染早期诊断中的应用。
Eur J Clin Microbiol. 1984 Apr;3(2):116-21. doi: 10.1007/BF02014328.
10
Enzyme-linked immunosorbent assay for serodiagnosis of Mycoplasma pneumoniae infections.用于肺炎支原体感染血清学诊断的酶联免疫吸附测定
J Clin Microbiol. 1983 Aug;18(2):432-5. doi: 10.1128/jcm.18.2.432-435.1983.
本文引用的文献
1
2
Immunochemical analysis of Mycoplasma pneumoniae. 3. Separation and chemical identification of serologically active lipids.肺炎支原体的免疫化学分析。3. 血清学活性脂质的分离与化学鉴定。
Aust J Exp Biol Med Sci. 1969 Apr;47(2):171-95. doi: 10.1038/icb.1969.19.
3
Immunochemical analysis of Mycoplasma pneumoniae.肺炎支原体的免疫化学分析。
Ann N Y Acad Sci. 1967 Jul 28;143(1):691-702. doi: 10.1111/j.1749-6632.1967.tb27715.x.
4
Antibodies to brain and other tissues in cases of Mycoplasma pneumoniae infection.肺炎支原体感染病例中针对脑及其他组织的抗体。
Clin Exp Immunol. 1971 Feb;8(2):319-33.
5
Isolation and characterization of fractions of Mycoplasma pneumoniae. II. Antigenicity and immunogenicity.肺炎支原体各组分的分离与特性鉴定。II. 抗原性与免疫原性。
J Bacteriol. 1966 Jun;91(6):2126-38. doi: 10.1128/jb.91.6.2126-2138.1966.
6
Immunogenicity of Mycoplasma pneumoniae glycolipids: a novel approach to the production of antisera to membrane lipids.肺炎支原体糖脂的免疫原性：一种制备抗膜脂抗血清的新方法。
Proc Natl Acad Sci U S A. 1970 Oct;67(2):590-7. doi: 10.1073/pnas.67.2.590.
7
Preparation of antigen for the indirect fluorescent antibody test in diagnosis of mycoplasma pneumoniae infection (A technical report).
Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(2):149-52. doi: 10.1111/j.1699-0463.1970.tb04281.x.
8
An indirect haemagglutination test for serum antibodies against mycoplasma pneumoniae using formalinized, tanned sheep erythrocytes.一种使用甲醛鞣制的绵羊红细胞检测血清中抗肺炎支原体抗体的间接血凝试验。
Acta Pathol Microbiol Scand. 1968;73(3):459-72. doi: 10.1111/j.1699-0463.1968.tb04615.x.
9
Antibody response in patients with acute pancreatitis to mycoplasma pneumoniae.急性胰腺炎患者对肺炎支原体的抗体反应。
Scand J Gastroenterol. 1973;8(7):631-5.
10
Inhibition of cold agglutinins (anti-I) by M. pneumoniae antigens.
Proc Soc Exp Biol Med. 1972 Feb;139(2):476-9. doi: 10.3181/00379727-139-36167.
Zotero 插件