Thomas J, Infantes-Lorenzo J A, Moreno I, Romero B, Garrido J M, Juste R, Domínguez M, Domínguez L, Gortazar C, Risalde M A
SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM), Ciudad Real, Spain; Indian Council of Agricultural Research (ICAR), New Delhi, India.
VISAVET Health Surveillance Centre, Complutense University of Madrid, Madrid, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Vet J. 2019 Feb;244:98-103. doi: 10.1016/j.tvjl.2018.12.021. Epub 2018 Dec 20.
Red deer (Cervus elaphus) farming is a growing economic activity worldwide. However, the capacity of this species to act as reservoir of animal tuberculosis (TB) poses a threat to other wildlife and to livestock. Diagnostic assay accuracy in this species is therefore highly relevant for prevention and control measures. Our aim was to evaluate the diagnostic performance of the protein complex P22, obtained from Mycobacterium bovis derived purified protein derivative (bPPD), as a candidate antigen for the detection of antibodies against Mycobacterium tuberculosis complex (MTC). We assessed the performance of this new antigen in indirect enzyme-linked immunosorbent assays (ELISA) in TB-positive and TB-negative red deer, in comparison with a bPPD-based ELISA. The P22 ELISA achieved a higher specificity (Sp) and similar sensitivity (Se) in comparison with the bPPD ELISA at all the cut-off points considered. The P22 ELISA yielded optimal Sp (99.02%; 95% confidence intervals [CI]: 96.5-99.8) and appropriate Se (70.1%; CI: 63.6-76) at the selected cut-off point of 100%. These results suggest that P22 can be used as an alternative antigen in the immunodiagnosis of animal TB through the use of an ELISA-type detection of antibodies against MTC in red deer, thus contributing to the diagnosis of animal TB in this species as a measure for further disease prevention and control programs.
马鹿（Cervus elaphus）养殖在全球范围内是一项不断发展的经济活动。然而，该物种作为动物结核病（TB）储存宿主的能力对其他野生动物和家畜构成了威胁。因此，该物种诊断检测的准确性对于预防和控制措施至关重要。我们的目的是评估从牛分枝杆菌衍生的纯化蛋白衍生物（bPPD）中获得的蛋白复合物P22作为检测结核分枝杆菌复合群（MTC）抗体的候选抗原的诊断性能。与基于bPPD的酶联免疫吸附测定（ELISA）相比，我们评估了这种新抗原在TB阳性和TB阴性马鹿间接ELISA中的性能。在所有考虑的临界值点，与bPPD ELISA相比，P22 ELISA具有更高的特异性（Sp）和相似的敏感性（Se）。在选定的100%临界值点，P22 ELISA产生了最佳的Sp（99.02%；95%置信区间[CI]：96.5 - 99.8）和适当的Se（70.1%；CI：63.6 - 76）。这些结果表明，P22可作为一种替代抗原，通过ELISA类型检测马鹿中抗MTC抗体用于动物TB的免疫诊断，从而有助于将该物种的动物TB诊断作为进一步疾病预防和控制计划的一项措施。
