Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan; Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Dent Mater. 2019 May;35(5):780-788. doi: 10.1016/j.dental.2019.02.019. Epub 2019 Mar 1.
Bioactive glass (BG) is widely used as a bioactive material for various clinical applications, and effective and efficient elemental release and an increase in mechanical strength are expected with further development. The purpose of this study is to clarify the physicochemical and biological characteristics of Sr-doped BG-incorporated glass ionomer cements.
Sr-doped BGs (45SiO-6PO-24.5NaO-(24.5-x)CaO-xSrO) (wt%), where × = 0, 6, 12, were prepared, and the particle size, crystallinity, and elemental release profiles were evaluated. The Sr-doped BGs were then incorporated into a glass ionomer cement at a weight ratio of 1:4, and the physicochemical properties (compressive strength, bending strength, hardness, and elemental release profile) were investigated. Cell attachment, cell proliferation, and osteoblastic differentiation were used to evaluate the biological characteristics.
The Sr-doped BGs were amorphous phases with a homogeneous particle size and exhibited sustained release of Ca, Si, and Sr. The BG-incorporated cements were able to release these elements while retaining the same mechanical properties as those of the pure glass ionomer cement. In addition, no cytotoxicity of osteoblasts or differences in the cell attachment or proliferation were observed for the BG-incorporated cements. In contrast, the Sr-doped BG-incorporated cements promoted the alkaline phosphatase activities of the osteoblasts without the need for any media supplements for osteoblastic differentiation.
Sr-releasable inorganic cements with high mechanical properties were successfully fabricated by incorporating Sr-doped BGs in glass ionomer cement. These bioactive materials are promising candidates for bone grafting materials, bone cements, and pulp capping materials.
生物活性玻璃（BG）被广泛用作各种临床应用的生物活性材料，并且期望通过进一步的发展实现有效和高效的元素释放以及机械强度的提高。本研究的目的是阐明掺锶 BG 玻璃离子水泥的物理化学和生物学特性。
制备了掺锶 BG（45SiO-6PO-24.5NaO-(24.5-x)CaO-xSrO）（wt%），其中×=0、6、12，评估了粒径、结晶度和元素释放曲线。然后将掺锶 BG 以 1:4 的重量比掺入玻璃离子水泥中，研究了物理化学性质（抗压强度、弯曲强度、硬度和元素释放曲线）。采用细胞黏附、细胞增殖和成骨细胞分化来评价生物特性。
掺锶 BG 为无定形相，具有均匀的粒径，并表现出 Ca、Si 和 Sr 的持续释放。BG 掺入的水泥能够释放这些元素，同时保持与纯玻璃离子水泥相同的机械性能。此外，对于掺 BG 的水泥，未观察到成骨细胞的细胞毒性或细胞黏附和增殖的差异。相反，掺 Sr 的 BG 水泥促进了成骨细胞的碱性磷酸酶活性，而无需任何介质补充来促进成骨细胞分化。
通过在玻璃离子水泥中掺入掺锶 BG，成功制备了具有高机械性能的可释放 Sr 的无机水泥。这些生物活性材料有望成为骨移植材料、骨水泥和牙髓盖髓材料的候选材料。
