锶/氟生物活性玻璃纳米颗粒对1.1%氟化钠牙膏的pH值、元素释放、牙本质再矿化及细胞毒性的影响
Effects of Sr/F-bioactive glass nanoparticles on pH, elemental release, dentin remineralisation, and cytotoxicity of 1.1% NaF toothpaste.
作者信息
Gesprasert Chananya, Kettratad Matana, Naruphontjirakul Parichart, Panpisut Piyaphong
机构信息
Dental Department, Prasat Hospital, Surin, Thailand.
Faculty of Dentistry, Thammasat University, Pathum Thani, Thailand.
出版信息
Biomater Investig Dent. 2025 Jul 22;12:44239. doi: 10.2340/biid.v12.44239. eCollection 2025.
OBJECTIVE
This study examined the effect of Sr/F-bioactive glass nanoparticles (Sr/F-BAG) concentration on 1.1% NaF toothpaste. The effects of additives on pH, fluoride and elemental release, dentin remineralisation, and cytotoxicity were determined.
MATERIALS AND METHODS
Sr/F-BAG particles were incorporated into 1.1% NaF toothpaste (0, 1, 2, and 4 wt%). F release and pH upon immersion in deionised water were determined using a fluoride-specific electrode and pH meter ( = 8). Elemental release was analysed using Inductively Coupled Plasma Optical Emission Spectroscopy ( = 3). Dentin remineralisation (mineral-to-collagen ratio) after application of experimental toothpaste was compared using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR, = 9). Cytotoxicity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay ( = 3). Colgate PreviDent 5000 Plus toothpaste (PV) was used as a commercial comparison.
RESULTS
The addition of 0 to 4 wt% Sr/F-BAG linearly increased pH and F release of the 1.1% NaF toothpaste. Each 1 wt% increase in Sr/F-BAG concentration, raised pH by 0.3 and fluoride release by 457 ppm. The additives also enhanced the release of Ca, P, and Sr from the experimental toothpaste. At high concentration of Sr/F-BAG (4 wt%), the pH of the experimental toothpaste was comparable to PV ( > 0.05) but with significantly higher fluoride release ( < 0.05). However, PV demonstrated a significantly higher increase in mineral-to-collagen ratio compared to the experimental materials. The dentin surface treated with PV also showed more evident mineral precipitation. Furthermore, the experimental toothpaste containing 4 wt% Sr/F-BAG demonstrated higher cell viability (90%) than PV (56%).
CONCLUSION
The addition of Sr/F-BAG enhanced the release of F, Ca, P, Sr, and increased the pH of the toothpaste. However, the experimental toothpaste with added bioactive glass up to 4 wt% did not demonstrate superior remineralising effects compared to commercial 1.1% NaF toothpaste. In addition, the incorporation of Sr/F-BAG promoted the cytocompatibility of the experimental toothpaste.
目的
本研究考察了锶/氟生物活性玻璃纳米颗粒（Sr/F-BAG）浓度对1.1%氟化钠牙膏的影响。测定了添加剂对pH值、氟化物和元素释放、牙本质再矿化及细胞毒性的影响。
材料与方法
将Sr/F-BAG颗粒加入1.1%氟化钠牙膏中（0、1、2和4 wt%）。使用氟离子特异性电极和pH计（n = 8）测定浸入去离子水中后的氟释放量和pH值。使用电感耦合等离子体发射光谱仪（n = 3）分析元素释放。使用衰减全反射傅里叶变换红外光谱仪（ATR-FTIR，n = 9）比较使用实验牙膏后牙本质的再矿化情况（矿物质与胶原蛋白的比例）。使用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐（MTT）法评估细胞毒性（n = 3）。高露洁PreviDent 5000 Plus牙膏（PV）用作商业对照。
结果
添加0至4 wt%的Sr/F-BAG可使1.1%氟化钠牙膏的pH值和氟释放量呈线性增加。Sr/F-BAG浓度每增加1 wt%，pH值升高0.3，氟释放量增加457 ppm。添加剂还增强了实验牙膏中钙、磷和锶的释放。在高浓度的Sr/F-BAG（4 wt%）下，实验牙膏的pH值与PV相当（P > 0.05），但氟释放量显著更高（P < 0.05）。然而，与实验材料相比，PV的矿物质与胶原蛋白比例增加显著更高。用PV处理的牙本质表面也显示出更明显的矿物质沉淀。此外，含有4 wt% Sr/F-BAG的实验牙膏显示出比PV（56%）更高的细胞活力（90%）。
结论
添加Sr/F-BAG可增强氟、钙、磷、锶的释放，并提高牙膏的pH值。然而，添加生物活性玻璃至4 wt%的实验牙膏与市售1.1%氟化钠牙膏相比，未显示出更好的再矿化效果。此外，加入Sr/F-BAG可提高实验牙膏的细胞相容性。
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