Houston Methodist Research Institute, Houston, TX, United States of America.
Center for Promotion of Advancement of Society (CPAS), Lot A4 15 Dong Quan, Cau Giay, Ha Noi, Vietnam.
PLoS One. 2019 Mar 4;14(3):e0213304. doi: 10.1371/journal.pone.0213304. eCollection 2019.
Although QuantiFERON-TB Gold Plus (QFT-Plus), a new interferon-gamma release assay, has shown good performance in adults, little data is available in children.
De-identified data from TB-suspected patients age <18 years with QFT-Plus results, who were admitted or screened at the National Lung Hospital (NLH) in Ha Noi, Vietnam in 2017, were assessed. Logistic regression analyses were performed to determine the characteristics associated with having a positive QFT-Plus result. Sensitivity, both overall and in subgroups of pulmonary TB only (PTB), extra-pulmonary TB (EPTB) only, and both PTB and EPTB were calculated.
Of 222 children with available QFT-Plus results, 33 were classified as confirmed TB, of whom 18 had QFT-Plus (+) and 15 had QFT-Plus (-). Multiple logistic regression modeling suggested that age, history of TB, and confirmed TB were significantly associated with having a positive QFT-Plus result with an area under the ROC curve of 0.77. QFT-Plus sensitivity in PTB only, EPTB, and both PTB and EPTB patients was 84.2%, 14.3% and 14.3%, respectively. The overall sensitivity of the QFT-Plus assay (regardless PTB or EPTB) in children was 54.5%.
Although QFT-Plus had a good sensitivity in children having exclusive PTB, it had poor sensitivity in EPTB.
新型干扰素-γ释放试验 QuantiFERON-TB Gold Plus（QFT-Plus）在成人中的表现良好，但在儿童中的数据较少。
对 2017 年在越南河内国家肺病医院（NLH）就诊或筛查的 QFT-Plus 结果为疑似结核病且年龄<18 岁的患者进行了 QFT-Plus 结果的回顾性分析。采用逻辑回归分析确定与 QFT-Plus 阳性结果相关的特征。计算了 QFT-Plus 的总体敏感性和仅肺结核（PTB）、仅肺外结核（EPTB）和 PTB 和 EPTB 同时存在时的敏感性。
在 222 名有 QFT-Plus 结果的儿童中，33 名被归类为确诊结核病，其中 18 名 QFT-Plus（+）和 15 名 QFT-Plus（-）。多变量逻辑回归模型显示，年龄、TB 病史和确诊 TB 与 QFT-Plus 阳性结果显著相关，ROC 曲线下面积为 0.77。QFT-Plus 在单纯 PTB、EPTB 和 PTB 和 EPTB 患者中的敏感性分别为 84.2%、14.3%和 14.3%。QFT-Plus 检测（无论是否存在 PTB 或 EPTB）在儿童中的总体敏感性为 54.5%。
尽管 QFT-Plus 在单纯肺结核儿童中具有良好的敏感性，但在肺外结核中的敏感性较差。
