Laboratory of Virology and Infectious Gastroenteritis, Pathology Department, Health Science Center, Federal University of Espírito Santo, Maruípe, Vitória 1468, ES, Brazil.
Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Rio de Janeiro 4365, RJ, Brazil.
Viruses. 2020 Sep 25;12(10):1084. doi: 10.3390/v12101084.
Host susceptibility according to human histo-blood group antigens (HBGAs) is widely known for norovirus infection, but is less described for rotavirus. Due to the variable HBGA polymorphism among populations, we aimed to evaluate the association between HBGA phenotypes (ABH, Lewis and secretor status) and susceptibility to rotavirus and norovirus symptomatic infection, and the polymorphisms of and , of children from southeastern Brazil. Paired fecal-buccal specimens from 272 children with acute diarrhea were used to determine rotavirus/norovirus genotypes and HBGAs phenotypes/genotypes, respectively. Altogether, 100 (36.8%) children were infected with rotavirus and norovirus. The rotavirus P[8] genotype predominates (85.7%). Most of the noroviruses (93.8%) belonged to genogroup II (GII). GII.4 Sydney represented 76% (35/46) amongst five other genotypes. Rotavirus and noroviruses infected predominantly children with secretor status (97% and 98.5%, respectively). However, fewer rotavirus-infected children were Lewis-negative (8.6%) than the norovirus-infected ones (18.5%). single nucleotide polymorphisms (SNP) occurred mostly at the T59G > G508A > T202C > C314T positions. Our results reinforce the current knowledge that secretors are more susceptible to infection by both rotavirus and norovirus than non-secretors. The high rate for Lewis negative (17.1%) and the combination of SNPs, beyond the secretor status, may reflect the highly mixed population in Brazil.
宿主对人类组织血型抗原（HBGAs）的易感性已广泛应用于诺如病毒感染，但在轮状病毒感染方面的描述较少。由于人群中 HBGAs 多态性的变化，我们旨在评估巴西东南部儿童的 HBGA 表型（ABH、Lewis 和分泌状态）与轮状病毒和诺如病毒症状性感染的易感性之间的关系，以及 和 的多态性。采集了 272 例急性腹泻儿童的粪便和口腔配对标本，分别用于确定轮状病毒/诺如病毒基因型和 HBGAs 表型/基因型。共有 100 例（36.8%）儿童感染了轮状病毒和诺如病毒。轮状病毒 P[8]基因型占主导地位（85.7%）。大多数诺如病毒（93.8%）属于基因群 II（GII）。GII.4 Sydney 在其他五种基因型中占 76%（35/46）。轮状病毒和诺如病毒主要感染分泌型儿童（分别为 97%和 98.5%）。然而，感染轮状病毒的儿童中 Lewis 阴性（8.6%）的比例低于感染诺如病毒的儿童（18.5%）。 单核苷酸多态性（SNP）主要发生在 T59G > G508A > T202C > C314T 位置。我们的结果强化了目前的知识，即分泌型儿童比非分泌型儿童更容易感染轮状病毒和诺如病毒。Lewis 阴性（17.1%）的高发生率以及 SNP 的组合，除了分泌状态外，可能反映了巴西高度混合的人口。
