Smith Denise R, Michailides Themis J, Stanosz Glen R
Department of Plant Pathology, University of Wisconsin, Madison 53706.
University of California, Davis, and Kearney Agricultural Center, Parlier, CA 93648.
Plant Dis. 2001 Dec;85(12):1235-1240. doi: 10.1094/PDIS.2001.85.12.1235.
A panicle and shoot blight disease of pistachio trees in California is caused by a fungus previously identified as the anamorph of Botryosphaeria dothidea. We have compared random amplified polymorphic DNA (RAPD) markers, nuclear rDNA internal transcribed spacer (ITS) region sequences, and conidium morphology of 15 isolates of the pistachio Fusicoccum to those of well-characterized isolates of B. dothidea, B. ribis, and F. luteum. Cluster analysis of RAPD markers separated the pistachio Fusicoccum isolates from B. dothidea, as did parsimony analysis of the ITS region sequences. Conidium size and shape were similar to those of B. ribis (Fusicoccum sp.) and F. luteum, but distinguishable from those of F. aesculi (the anamorph of B. dothidea). We conclude that the fungus causing panicle and shoot blight of pistachio is distinguishable from B. dothidea and is part of a complex containing B. ribis, F. luteum, and other fungi with Fusicoccum anamorphs.
加利福尼亚开心果树上的一种穗枯和枝枯病是由一种先前被鉴定为葡萄座腔菌(Botryosphaeria dothidea)无性型的真菌引起的。我们比较了15株开心果壳梭孢菌(Fusicoccum)分离株的随机扩增多态性DNA(RAPD)标记、核糖体DNA内部转录间隔区(ITS)序列以及分生孢子形态,与特征明确的葡萄座腔菌、茶藨子葡萄座腔菌(B. ribis)和黄斑壳梭孢菌(F. luteum)分离株进行了比较。RAPD标记的聚类分析将开心果壳梭孢菌分离株与葡萄座腔菌区分开来,ITS区域序列的简约分析也是如此。分生孢子的大小和形状与茶藨子葡萄座腔菌(壳梭孢菌属物种)和黄斑壳梭孢菌相似,但与七叶树壳梭孢菌(葡萄座腔菌的无性型)不同。我们得出结论,引起开心果穗枯和枝枯病的真菌与葡萄座腔菌不同,是包含茶藨子葡萄座腔菌、黄斑壳梭孢菌以及其他具有壳梭孢菌无性型的真菌的复合体的一部分。