Guarino Salvatore, Mercati Francesco, Fatta Del Bosco Sergio, Motisi Antonio, Abbate Loredana
Institute of Biosciences and Bioresources (IBBR), National Research Council of Italy (CNR), Corso Calatafimi 414, 90129 Palermo, Italy.
Plants (Basel). 2022 Dec 8;11(24):3426. doi: 10.3390/plants11243426.
The citrus tristeza virus (CTV) is an agent of devastating epidemics of the citrus plant grafted on one of the main rootstocks still used in the Mediterranean area. Consequently, CTV-tolerant alternative citrus rootstocks are considered necessary to manage this disease and/or its vector; that in Mediterranean countries is the aphid . In this study, we analyzed the VOCs emitted from plants grafted on the CTV-susceptible and on the CTV-tolerant Volkamer lemon, Forner-Alcaide no. 5, and Carrizo citrange. Furthermore, the aphid preference/avoidance response toward these combinations was evaluated in a semi-field experiment. The VOC profiles recorded on the leaves of grafted on the four rootstocks listed above showed significant differences in the abundances and ratios of the compounds emitted. The behavioral experiments indicated that prefers to orient and establish on the plants grafted on rather than on that grafted on the three CTV-tolerant varieties. The possibility that this avoidance mechanism is triggered by the different profile of the VOC emitted by the different combinations and the consequent susceptibility/tolerance shown toward CTV is discussed.
柑橘衰退病毒（CTV）是一种能引发毁灭性疫情的病原体，可感染嫁接在地中海地区仍在使用的主要砧木之一上的柑橘类植物。因此，人们认为有必要使用耐CTV的替代柑橘砧木来防治这种病害和/或其传播媒介；在地中海国家，这种传播媒介是蚜虫。在本研究中，我们分析了嫁接在对CTV敏感和耐CTV的伏尔卡默柠檬、福尔纳-阿尔凯德5号和卡里佐枳橙上的植物所释放的挥发性有机化合物（VOCs）。此外，在半田间试验中评估了蚜虫对这些组合的偏好/回避反应。记录在上述四种砧木上嫁接的植物叶片上的VOCs谱图显示，所释放化合物的丰度和比例存在显著差异。行为实验表明，蚜虫更喜欢在嫁接在[未提及砧木名称]上的植物上定位和栖息，而不是在嫁接在三种耐CTV品种上的植物上。本文讨论了这种回避机制是否由不同组合所释放的VOCs的不同谱图以及由此表现出的对CTV的易感性/耐受性所触发的可能性。
