Department of Bionanosystem Engineering, Chonbuk National University, Jeonju, Jeonbuk, South Korea; Department of Medical Practicing, Woori Convalescent Hospital, Jeonju, Jeonbuk, South Korea.
Department of Bionanosystem Engineering, Chonbuk National University, Jeonju, Jeonbuk, South Korea.
Carbohydr Polym. 2019 May 15;212:21-29. doi: 10.1016/j.carbpol.2019.01.085. Epub 2019 Feb 11.
Synthetic polymers are easy to process and have excellent mechanical properties but low wettability and poor cell compatibility limit their applications in tissue scaffolding. In this study, a facile procedure was established to regenerate cellulose and calcium lactate (CaL) into a polycaprolactone (PCL) nanofibrous scaffold for tissue engineering applications. Briefly, varying amounts of lactic acid (LA) was mixed with the blend of PCL and cellulose acetate (CA) solutions and electrospun to fabricate an optimal composite PCL/CA/LA fibrous membrane. Later on, as-prepared membranes were treated with calcium hydroxide solution. This process simultaneously converted CA and LA contents into Cellulose and CaL, respectively. In situ regeneration of Cellulose and CaL into the composite fiber remarkably enhanced the biological and physicochemical properties of the composite fiber. This work provides a novel dual-channel strategy for simultaneous regeneration of biopolymer and bioactive molecule into the PCL nanofiber for regenerative medicine and tissue engineering applications.
合成聚合物易于加工，具有优异的机械性能，但低润湿性和较差的细胞相容性限制了它们在组织支架中的应用。在这项研究中，建立了一种简便的方法，将纤维素和乳酸钙（CaL）再生为聚己内酯（PCL）纳米纤维支架，用于组织工程应用。简要地说，将不同量的乳酸（LA）与 PCL 和醋酸纤维素（CA）溶液的混合物混合，并进行静电纺丝以制造最佳的复合 PCL/CA/LA 纤维膜。之后，将制备好的膜用氢氧化钙溶液处理。该过程将 CA 和 LA 含量分别同时转化为纤维素和 CaL。纤维素和 CaL 的原位再生显著提高了复合纤维的生物和物理化学性能。这项工作为同时将生物聚合物和生物活性分子再生到 PCL 纳米纤维中提供了一种新颖的双通道策略，用于再生医学和组织工程应用。
