Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju, Republic of Korea.
Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju, Republic of Korea; Department of Bionanotechnology and Bioconvergence Engineering, Graduate School, Jeonbuk National University, Jeonju, Republic of Korea.
Carbohydr Polym. 2021 Feb 1;253:117191. doi: 10.1016/j.carbpol.2020.117191. Epub 2020 Oct 9.
Cellulose and its derivatives are widely used as nanofibrous biomaterials, but obtaining 3D cellulose nanofibers is difficult and relevant research is scarce. In the present study, we propose a simple method for converting electrospun 3D cellulose acetate/lactic acid nanofibers via calcium hydroxide treatment into a 3D cellulose/calcium lactate nanocomposite matrix. The conversion resulted in producing a stronger nanofibrous matrix (1.382 MPa vs. 0.112 MPa) that is more hydrophilic and cell-friendly compared to the untreated cellulose acetate/lactic acid group. The successful conversion was verified via FTIR, XPS, TGA, DTG, and XRD. The ability of the scaffolds to provide a suitable environment for cell growth and infiltration was verified by CCK assay and confocal microscopy. The porous nature, mechanical strength, and presence of calcium make the 3D cellulose/calcium lactate matrix a promising material for bone tissue engineering.
纤维素及其衍生物被广泛用作纳米纤维生物材料，但获得 3D 纤维素纳米纤维较为困难，相关研究也较少。在本研究中，我们提出了一种简单的方法，通过氢氧化钙处理将静电纺丝的 3D 醋酸纤维素/乳酸纳米纤维转化为 3D 纤维素/乳酸钙纳米复合材料基质。与未经处理的醋酸纤维素/乳酸组相比，这种转化产生了更强的纳米纤维基质（1.382 MPa 对 0.112 MPa），具有更好的亲水性和细胞友好性。通过 FTIR、XPS、TGA、DTG 和 XRD 验证了转化的成功。CCK 测定和共聚焦显微镜验证了支架为细胞生长和渗透提供合适环境的能力。多孔性、机械强度和钙的存在使 3D 纤维素/乳酸钙基质成为骨组织工程有前途的材料。
