绿茎穿心莲（Kalmegh）中一种新阿拉伯木聚糖的结构和抗氧化研究。
Structural and antioxidant studies of a new arabinoxylan from green stem Andrographis paniculata (Kalmegh).
机构信息
Department of Microbiology, Panskura Banamali College, Panskura, Purba Midnapore, 721152, West Bengal, India.
Department of Chemistry, Panskura Banamali College, Panskura, Purba Midnapore, 721152, West Bengal, India; Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India.
出版信息
Carbohydr Polym. 2019 May 15;212:297-303. doi: 10.1016/j.carbpol.2019.02.051. Epub 2019 Feb 18.
A water soluble arabinoxylan (APPS), with a molecular weight ∼1.49 × 10 Da was isolated from the green stem of Andrographis paniculata. The APPS contained D-xylose, 2-methoxy D-xylose and L- arabinose in molar ratio of 3:1:1. The structure of the repeating unit in the polysaccharide was determined through several chemical (acid hydrolysis, Methylation analysis and periodate oxidation) and spectroscopic analysis (1D/2D NMR experiments) as: In vitro antioxidant assay, APPS was found to possess ferrous ion chelating activity (EC = 283 μg/ml), superoxide radical scavenging activity (EC = 470 μg/ml), and hydroxide radical scavenging activity (EC = 193 μg/ml). Thus, APPS could be used as a natural antioxidant agent for food and pharmaceutical industries.
从穿心莲的绿色茎中分离出一种水溶性阿拉伯木聚糖（APPS），其分子量约为 1.49×10 Da。APPS 含有 D-木糖、2-甲氧基 D-木糖和 L-阿拉伯糖，摩尔比为 3:1:1。多糖重复单元的结构通过几种化学（酸水解、甲基化分析和高碘酸盐氧化）和光谱分析（1D/2D NMR 实验）确定为：体外抗氧化活性测定，APPS 具有亚铁离子螯合活性（EC = 283μg/ml）、超氧自由基清除活性（EC = 470μg/ml）和羟基自由基清除活性（EC = 193μg/ml）。因此，APPS 可用作食品和制药工业的天然抗氧化剂。
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