Key Laboratory of Standardization of Chinese Medicines of the Ministry of Education, Shanghai, PR China.
Pharm Biol. 2010 May;48(5):589-94. doi: 10.3109/13880200903214231.
An activity-directed fractionation and purification process was used to isolate 1,1-diphenyl-2-picrylhydrazyl radical (DPPH*) scavenging components from fruits of Capparis spinosa L. (Capparaeae). Ethyl acetate and aqueous fractions showed greater DPPH* scavenging activities compared to the petroleum ether fractions. The ethyl acetate fraction was subjected to purification using column chromatography. A new antioxidant cappariside (4-hydroxy-5-methylfuran-3-carboxylic acid, 1), together with seven known organic acids (2-8) for the first time from plants of genus Capparis and four known organic acids (9-12) were isolated from C. spinosa. The structures were elucidated by extensive analysis of 1D- and 2D-NMR spectroscopic. In addition, compounds 1, 2, 4, 5, 9, 10 and 12 indicated strong scavenging capacity for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals with a SC(50) value of 0.204 +/- 0.002, 0.007 +/- 0.0, 0.011 +/- 0.0, 0.044 +/- 0.0016, 0.032 +/- 0.0, 0.090 +/- 0.001, and 0.350 +/- 0.017 mM, respectively.
采用活性导向的分步提取和纯化方法，从药用植物仙人掌果实中分离得到 1,1-二苯基-2-苦基肼自由基（DPPH*）清除成分。乙酸乙酯和水相部分比石油醚部分具有更强的 DPPH*清除活性。对乙酸乙酯部分进行柱层析纯化。首次从山柑属植物中分离得到一种新的抗氧化剂山柑子苷（4-羟基-5-甲基呋喃-3-羧酸，1），以及 7 种已知有机酸（2-8）和 4 种已知有机酸（9-12）。通过对 1D 和 2D-NMR 光谱的广泛分析，确定了这些化合物的结构。此外，化合物 1、2、4、5、9、10 和 12 对 1,1-二苯基-2-苦基肼（DPPH）自由基具有很强的清除能力，SC(50) 值分别为 0.204 +/- 0.002、0.007 +/- 0.0、0.011 +/- 0.0、0.044 +/- 0.0016、0.032 +/- 0.0、0.090 +/- 0.001 和 0.350 +/- 0.017 mM。
