State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China.
State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China; Biological Science Research Center, Southwest University, Beibei, Chongqing 400715, China; Chongqing Key Laboratory of Sericultural Science, Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Beibei, Chongqing 400715, China.
Carbohydr Polym. 2019 May 15;212:403-411. doi: 10.1016/j.carbpol.2019.02.069. Epub 2019 Feb 22.
Developing biomaterials based on the natural biomacromolecule silk sericin from Bombyx mori cocoon is of great interest for biomedical application. Dialdehyde carboxymethyl cellulose (DCMC) is derived from periodate oxidation of carboxy- methyl cellulose. Here, we developed a novel strategy of cross-linking of sericin with DCMC via the Schiff's base reaction. Fourier transform infrared spectroscopy and scanning electron microscopy indicated the formation of Schiff's base via the blending of sericin and DCMC. The mechanical properties tests suggested the covalent cross-linking effectively enhanced the tensile strength of sericin. The swelling test and water contact angle indicated the DCMC/SS film had excellent hydrophilicity, swellability. Additionally, we demonstrated the DCMC/SS film had excellent blood compatibility, cytocompatibility and promoting cell proliferation activity by the hemolysis ratio analysis, cell adhesion, cells viability and proliferation assays. The prepared DCMC/SS film has shown great promise in biomedical applications such as wound dressing, artificial skin and tissue engineering.
以家蚕茧中的天然生物大分子丝胶为基础开发生物材料，对于生物医学应用具有重要意义。二醛羧甲基纤维素（DCMC）是通过过碘酸盐氧化羧甲基纤维素得到的。在这里，我们通过席夫碱反应开发了一种将丝胶与 DCMC 交联的新策略。傅里叶变换红外光谱和扫描电子显微镜表明，丝胶和 DCMC 的共混形成了席夫碱。力学性能测试表明，共价交联有效地提高了丝胶的拉伸强度。溶胀试验和水接触角表明，DCMC/SS 薄膜具有优异的亲水性和溶胀性。此外，通过溶血率分析、细胞黏附、细胞活力和增殖试验，我们证明了 DCMC/SS 薄膜具有优异的血液相容性、细胞相容性和促进细胞增殖活性。所制备的 DCMC/SS 薄膜在伤口敷料、人工皮肤和组织工程等生物医学应用中具有广阔的应用前景。
