Dai Jianhua, Wang Minyan, Yin Hengfu, Han Xiaojiao, Fan Yanru, Wei Yi, Lin Jie, Liu Jun
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, China.
Front Plant Sci. 2024 May 29;15:1378418. doi: 10.3389/fpls.2024.1378418. eCollection 2024.
The strong aromatic characteristics of the tender leaves of determine their quality and economic value.
Here, GC-MS analysis revealed that caryophyllene is a key volatile compound in the tender leaves of two different varieties, however, the transcriptional mechanisms controlling its gene expression are unknown. Comparative transcriptome analysis revealed significant enrichment of terpenoid synthesis pathway genes, suggesting that the regulation of terpenoid synthesis-related gene expression is an important factor leading to differences in aroma between the two varieties. Further analysis of expression levels and genetic evolution revealed that is a caryophyllene synthase, which was confirmed by transient overexpression in and leaves. Furthermore, we screened an AP2/ERF transcriptional factor ERF-IX member, , for the potential regulation of caryophyllene synthesis. The had a similar expression trend to that of and was highly expressed in high-aroma varieties and tender leaves. Exogenous spraying of MeJA also induced the expression of and and promoted the biosynthesis of caryophyllene. Transient overexpression of in T. sinensis significantly promoted expression and caryophyllene biosynthesis.
Our results showed that promoted the expression of and the biosynthesis of caryophyllene in leaves, providing a strategy for improving the aroma of tender leaves.
嫩叶强烈的芳香特性决定了它们的品质和经济价值。
在此，气相色谱 - 质谱联用（GC - MS）分析表明，石竹烯是两个不同品种嫩叶中的关键挥发性化合物，然而，控制其基因表达的转录机制尚不清楚。比较转录组分析显示萜类合成途径基因显著富集，表明萜类合成相关基因表达的调控是导致两个品种香气差异的重要因素。对表达水平和基因进化的进一步分析表明，[具体名称未给出]是一种石竹烯合酶，这在[具体植物名称未给出]和[具体植物名称未给出]叶片中的瞬时过表达得到了证实。此外，我们筛选了一个AP2/ERF转录因子ERF - IX成员[具体名称未给出]，用于对石竹烯合成的潜在调控。[具体名称未给出]与[具体名称未给出]具有相似的表达趋势，并且在高香气品种和嫩叶中高表达。外源喷施茉莉酸甲酯（MeJA）也诱导了[具体名称未给出]和[具体名称未给出]的表达，并促进了石竹烯的生物合成。[具体名称未给出]在中华[具体植物名称未给出]中的瞬时过表达显著促进了[具体名称未给出]的表达和石竹烯的生物合成。
我们的结果表明，[具体名称未给出]促进了[具体植物名称未给出]叶片中[具体名称未给出]的表达和石竹烯的生物合成，为改善嫩叶香气提供了一种策略。
