Paulista State University (Unesp)Universidade Estadual Paulista (Unesp), Faculty of Agrarian and Veterinary Sciences, Jaboticabal, SP, Brazil.
Paulista State University (Unesp)Universidade Estadual Paulista (Unesp), Faculty of Agrarian and Veterinary Sciences, Jaboticabal, SP, Brazil; Directorate of Animal Sciences, Maputo, Mozambique.
Ticks Tick Borne Dis. 2019 Apr;10(3):651-664. doi: 10.1016/j.ttbdis.2019.02.012. Epub 2019 Feb 26.
Although species of Anaplasma are highly prevalent Rickettsiales agents in domestic and wild ruminants with a wide distribution worldwide, few studies have been conducted so far to detect and/or investigate the diversity of these agentsin cattle in Mozambique. In the present study, serological and molecular assays were used to investigate the occurrence of Anaplasma spp. in 219 bovines sampled in the districts of Boane, Magude, Matutuíne, Moamba and Namaacha in Maputo, Mozambique. In the iELISA test for detection ofIgG antibodies to A. marginale, 86.3% (189/219) of the samples were positive. In qPCR assays for the gene msp1β for A. marginale and msp2 for A. phagocytophilum, 97.3% (213/219) and 2.7% (6/219) of the animals were positive, respectively. Two different cPCR protocols based on the 16S rRNA gene showed that 100% of the samples were positive for Anaplasma spp. The DNA sequences obtained were phylogenetically related to A. platys, A. phagocytophilum, Candidatus Anaplasma boleense, A. centrale, A. marginale and A. ovis. Phylogenetic inference based on the msp4 and msp5 genes positioned the obtained sequences in the clade of A. marginale, with evidence of occurrence of 8 and 5 different haplotypes for each gene, respectively. Anaplasma sp. phylogenetically associated with A. platys was evidenced in phylogenetic analyzes based on 16S rRNA and groEL genes. It is concluded that a high diversity of species of Anaplasma spp. occurs in cattle in Mozambique.
虽然在全球范围内，绵羊和山羊中有许多种安氏立克次体是普遍存在的立克次体病原体，但迄今为止，在莫桑比克对这些病原体在牛中的检测和/或多样性进行的研究很少。在本研究中，使用血清学和分子检测方法调查了莫桑比克马普托省博阿内、马古塔蒂尼、马图蒂内、莫阿巴和纳马沙区的 219 头牛中安氏立克次体的发生情况。在检测针对 A. marginale 的 IgG 抗体的 iELISA 检测中，86.3%（189/219）的样本呈阳性。在针对 A. marginale 的 msp1β基因和 A. phagocytophilum 的 msp2 基因的 qPCR 检测中，97.3%（213/219）和 2.7%（6/219）的动物呈阳性。基于 16S rRNA 基因的两种不同的 cPCR 方案显示，100%的样本对安氏立克次体呈阳性。获得的 DNA 序列在系统发育上与 A. platys、A. phagocytophilum、Candidatus Anaplasma boleense、A. centrale、A. marginale 和 A. ovis 有关。基于 msp4 和 msp5 基因的系统发育推断将获得的序列定位在 A. marginale 的分支中，每个基因分别存在 8 个和 5 个不同的单倍型。在基于 16S rRNA 和 groEL 基因的系统发育分析中，证实了与 A. platys 系统发育相关的安氏立克次体。结论是，在莫桑比克的牛中存在高度多样化的安氏立克次体。
