Institute of Forensic Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, China.
Sci Rep. 2019 Mar 4;9(1):3371. doi: 10.1038/s41598-019-39794-y.
Short tandem repeats (STRs) with features of high polymorphism and abundant evolution information play a significant role in genetic applications such as human forensics, anthropology and population genetics. The Huaxia Platinum System was specifically exploited to allow coamplification of all markers in the expanded Combined DNA Index System and the Chinese National Database. Herein, in continuation of our previous studies, 493 unrelated individuals were firstly genotyped to investigate the efficacy of this novel system in three minority ethnicities of China (Hui, Tibetan and Uygur). Additionally, genetic relationships among our three investigated populations and other previously published populations were analyzed using pairwise genetic distances, multidimensional scaling (MDS), principal component analysis (PCA), cladogram and STRUCTURE. The combined match probabilities (CMP) for the Hui, Tibetan and Uygur groups were 1.6894 × 10, 6.1666 × 10 and 5.0655 × 10, respectively, and the combined powers of exclusion (CPE) were 0.999999999646627, 0.999999999304935 and 0.999999999433994. Population comparison analysis manifested that the Hui and Tibetan populations had genetic affinities with the Han, Yi and Korean populations, while the Uygur group had a close relationship with the Kazakh population. The aforementioned results suggested that the Huaxia Platinum System is a polymorphic and effective tool that is appropriate for personal identification and population genetics.
短串联重复序列（STRs）具有高度多态性和丰富的进化信息特征，在遗传应用中发挥着重要作用，如人类法医学、人类学和群体遗传学。华夏铂金系统专门用于允许扩增扩展的联合 DNA 索引系统和中国国家数据库中的所有标记。在此，继我们之前的研究之后，首先对 493 名无关个体进行基因分型，以研究该新系统在中国三个少数民族（回族、藏族和维吾尔族）中的功效。此外，还使用成对遗传距离、多维尺度分析（MDS）、主成分分析（PCA）、系统发育树和 STRUCTURE 分析了我们三个研究人群与其他先前发表人群之间的遗传关系。回族、藏族和维吾尔族的联合匹配概率（CMP）分别为 1.6894×10、6.1666×10 和 5.0655×10，联合排除概率（CPE）分别为 0.999999999646627、0.999999999304935 和 0.999999999433994。群体比较分析表明，回族和藏族与汉族、彝族和朝鲜族具有遗传亲缘关系，而维吾尔族与哈萨克族关系密切。上述结果表明，华夏铂金系统是一种多态性和有效的工具，适用于个人识别和群体遗传学。
