Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, 610041, China.
Department of Criminal Science and Technology, People's Public Security University of China, Beijing, 100038, China.
Sci Rep. 2018 Feb 5;8(1):2429. doi: 10.1038/s41598-018-20871-7.
Short tandem repeats (STRs) are attractive to genetic applications like forensic, anthropological and population genetics studies. The Huaxia Platinum System was specifically developed to allow co-amplification and detection of all markers in the expanded CODIS core loci and the Chinese National Database. In this study, in continuation to our previous validation study, 568 unrelated individuals were firstly genotyped to investigate the effectiveness of this novel assay in 3 main ethnic groups of China (Han, Tibetan and Yi). The combined power of discrimination (CPD) were 0.9999999999999999999999999992, 0.999999999999999999999999992, 0.999999999999999999999999998, respectively, and the combined power of exclusion (CPE) were 0.9999999999, 0.999999995, 0.999999998, respectively. Next, genetic relationships along administrative and ethnic divisions were analyzed using pairwise genetic distances, multidimensional scaling (MDS), principal component analysis (PCA) and phylogenetic analysis. The Han ethnicity showed a high genetic homogeneity all across China, and significant genetic differences existed between Han groups and some minority groups, most prominently for the Tibetans, the Uyghurs, the Kazakhs, the Miaos, the Zhuangs and the Dais. Aforementioned results suggested that the Huaxia Platinum System is polymorphic and informative, which provides an efficient tool not only for human forensics, but also for population genetics studies.
短串联重复序列（STRs）在法医、人类学和群体遗传学研究等遗传应用中很有吸引力。华夏铂金系统是专门开发的，允许对 CODIS 核心基因座和中国国家数据库中所有标记进行共同扩增和检测。在这项研究中，继我们之前的验证研究之后，首先对 568 名无关个体进行基因分型，以研究该新型检测方法在中国 3 个主要民族（汉族、藏族和彝族）中的有效性。合并判别力（CPD）分别为 0.999999999999999999999999992、0.999999999999999999999999992、0.999999999999999999999999998，合并排除力（CPE）分别为 0.9999999999、0.999999995、0.999999998。接下来，使用成对遗传距离、多维尺度分析（MDS）、主成分分析（PCA）和系统发育分析分析行政和种族划分的遗传关系。汉族在中国各地表现出高度的遗传同质性，汉族群体与一些少数民族群体之间存在显著的遗传差异，其中藏族、维吾尔族、哈萨克族、苗族、壮族和傣族最为明显。上述结果表明，华夏铂金系统具有多态性和信息量，不仅为法医学提供了有效的工具，也为群体遗传学研究提供了有效的工具。
