Schreier P H, Bothwell A L, Mueller-Hill B, Baltimore D
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4495-9. doi: 10.1073/pnas.78.7.4495.
To compare the structure of IgG2a alleles we have determined the complete DNA sequence of the constant region, coding sequence, and 3' untranslated region of a cDNA clone, pAB gamma 2a-1, which was derived from the C57BL/6 mouse strain (b allotype). This sequence was compared with the corresponding IgG2a DNA sequence of BALB/c origin (a allotype). The DNA sequences showed 10% differences, and the deduced protein sequences differed by about 15%. These differences were not evenly distributed: most differences were in the hinge region, the CH3 domain and the 3' untranslated region. It is evident that many alterations in the IgG2a alleles have occurred since the a and b haplotypes were separated--some of these changes were point mutations but some appear to have resulted from gene conversion of the IgG2ab allele by the IgG2bb allele.
为了比较IgG2a等位基因的结构，我们测定了一个cDNA克隆pABγ2a - 1恒定区、编码序列和3'非翻译区的完整DNA序列，该克隆源自C57BL/6小鼠品系（b同种异型）。将此序列与源自BALB/c（a同种异型）的相应IgG2a DNA序列进行比较。DNA序列显示出10%的差异，推导的蛋白质序列差异约为15%。这些差异分布不均：大多数差异存在于铰链区、CH3结构域和3'非翻译区。显然，自a和b单倍型分离以来，IgG2a等位基因发生了许多改变——其中一些变化是点突变，但有些似乎是由IgG2bb等位基因对IgG2ab等位基因的基因转换导致的。
