Department of Otorhinolaryngology Head and Neck Surgery, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong, China.
Department of Otolaryngology, Shandong Energy Zibo Mining Group Co, Ltd General Hospital, Zibo, Shandong, China.
J Cell Physiol. 2019 Aug;234(10):17912-17924. doi: 10.1002/jcp.28424. Epub 2019 Mar 4.
This study aims to investigate the role of miR-144-3p and phosphatase and tensin homolog (PTEN) in nasopharyngeal carcinoma (NPC), along with their crosstalk with the phosphoinositide 3-kinase (PI3K)-protein kinase B (Akt) pathway.
Quantitative reverse transcription polymerase chain reaction and western blot were used to measure the gene expression at the transcriptional and translational levels. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and colony formation assay were used to examine cell proliferation via standard protocol. Transwell assay was conducted to examine cell invasiveness. A flow cytometer was used to determine cell apoptosis. Dual-Luciferase Reporter Gene Assay (SLDL-100) was used to confirm the target relationship between miR-144-3p and PTEN. Xenografts were used to detect the in vivo effects of the molecules of interest.
miR-144-3p was significantly overexpressed, whereas PTEN was more underexpressed in tumor tissues than in adjacent tissues. miR-144-3p promoted the proliferation and invasion of NPC cells and inhibited apoptosis by directly targeting PTEN, which improves PI3K-Akt signaling. miR-144-3p forced epithelial-mesenchymal transition in NPC.
miR-144-3p promotes the progression of NPC by directly targeting PTEN via crosstalk with PI3K-Akt signaling.
本研究旨在探讨 miR-144-3p 和磷酸酶张力蛋白同源物（PTEN）在鼻咽癌（NPC）中的作用，以及它们与磷酸肌醇 3-激酶（PI3K）-蛋白激酶 B（Akt）通路的相互作用。
采用定量逆转录聚合酶链反应和蛋白质印迹法检测基因在转录和翻译水平上的表达。采用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）比色法和集落形成实验按照标准方案检测细胞增殖。采用 Transwell 实验检测细胞侵袭能力。采用流式细胞仪检测细胞凋亡。双荧光素酶报告基因检测（SLDL-100）用于验证 miR-144-3p 和 PTEN 之间的靶标关系。利用异种移植检测感兴趣分子的体内作用。
miR-144-3p 在肿瘤组织中明显过表达，而 PTEN 在肿瘤组织中的表达明显低于相邻组织。miR-144-3p 通过直接靶向 PTEN 促进 NPC 细胞的增殖、侵袭和抑制凋亡，从而增强 PI3K-Akt 信号通路。miR-144-3p 可诱导 NPC 发生上皮-间充质转化。
miR-144-3p 通过与 PI3K-Akt 信号通路的相互作用，直接靶向 PTEN 促进 NPC 的进展。
