Luo Dong, Wang Wei, Chen Junlin, Liu Baoru, Chen Jinyun, Wang Yan, Chen Wenzhi
State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science and Technol-ogy, College of Biomedical Engineering, Chongqing Key Laboratory of Biomedical Engineering; Chongqing Medical University, Chongqing Collaborative Innovation Center for Minimally-invasive and Noninvasive Medicine, Chongqing, 400016.
Nan Fang Yi Ke Da Xue Xue Bao. 2019 Jul 30;39(7):836-842. doi: 10.12122/j.issn.1673-4254.2019.07.14.
To investigate the effect of low-intensity pulsed ultrasound (LIPUS) on hematopoietic function in rats after combined chemotherapy with doxorubicin and cyclophosphamide.
Eighty rats were randomized into control group and LIPUS group (=40) for treatment with intraperitoneal injection of doxorubicin (2 mg/kg)+cyclophosphamide (20 mg/kg) for 4 consecutive days and continuous irradiation with LIPUS for 7 days following the injections, respectively. The white blood cells, red blood cells and platelets counts in each group were measured at 0, 4, 7, 9, 11, 14 and 18 days after the start of drug administration. The pathological sections of the bone marrow were examined at 0, 4 and 11 days, and the flow cytometry was performed for detecting the cell apoptosis; qPCR was performed for detecting the expressions of SCF, ICAM-1, and VCAM-1 mRNAs, and ELISA was used to detect the expressions of IL-3 and GM-CSF.
The white blood cell count was significantly higher in LIPUS group than in the control group ( < 0.05). Histopathological examination of the bone marrow revealed significantly increased hematopoietic tissue in LIPUS group ( < 0.05). Flow cytometry demonstrated an obviously lower cell apoptosis rate in the bone marrow in LIPUS group than in the control group ( < 0.05). Compared with those in the control group, the mRNA expression levels of ICAM-1 and VCAM-1 as well as the protein levels of IL-3 and GM-CSF were significantly increased in LIPUS group ( < 0.05).
LIPUS can alleviate the hematopoietic damage after combined chemotherapy with doxorubicin with cyclophosphamide probably by increasing the expressions of ICAM- 1, VCAM-1, IL- 3, and GM-CSF.
探讨低强度脉冲超声（LIPUS）对阿霉素和环磷酰胺联合化疗后大鼠造血功能的影响。
将80只大鼠随机分为对照组和LIPUS组（每组40只），分别腹腔注射阿霉素（2mg/kg）+环磷酰胺（20mg/kg），连续4天，注射后分别连续7天用LIPUS照射。给药开始后0、4、7、9、11、14和18天检测每组的白细胞、红细胞和血小板计数。在0、4和11天检查骨髓病理切片，进行流式细胞术检测细胞凋亡；进行qPCR检测SCF、ICAM-1和VCAM-1 mRNA的表达，用ELISA检测IL-3和GM-CSF的表达。
LIPUS组白细胞计数明显高于对照组（P<0.05）。骨髓组织病理学检查显示LIPUS组造血组织明显增加（P<0.05）。流式细胞术显示LIPUS组骨髓细胞凋亡率明显低于对照组（P<0.05）。与对照组相比，LIPUS组ICAM-1和VCAM-1的mRNA表达水平以及IL-3和GM-CSF的蛋白水平明显升高（P<0.05）。
LIPUS可能通过增加ICAM-1、VCAM-1、IL-3和GM-CSF的表达来减轻阿霉素与环磷酰胺联合化疗后的造血损伤。
