Ganbold Boldbayar, Kim Seong-Kyun, Heo Seong-Joo, Koak Jai-Young, Lee Zang Hee, Cho Jaejin
Department of Prosthodontics and Dental Research Institute, School of Dentistry, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea.
Department of Prosthodontics and Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea.
Materials (Basel). 2019 Mar 4;12(5):732. doi: 10.3390/ma12050732.
Zirconia is worth studying as an alternative to dental titanium implants to overcome the disadvantages of titanium. This study investigated the surface characteristics of the zirconia implant material and osteoclastogenesis responses on the surface compared with titanium. Yttrium oxide-stabilized 5% tetragonal zirconia polycrystalline specimens were manufactured, and osteoclast-precursor cells were cultured and differentiated into osteoclasts on the specimens. Surface shape, roughness, and chemical composition were evaluated. After culturing, cell morphologies and differentiation capacity were analyzed using tartrate-resistant acid phosphatase activity (TRACP). mRNA of two critical transcription factors, nuclear factor of activated T-cells 1 () and were measured, and protein levels of and were investigated. The zirconia specimens had rhomboid-like shapes with smooth surfaces and exhibited no difference in surface roughness compared to the titanium specimens. Morphologies of differentiated osteoclasts on both materials were similar. TRACP activity on the zirconia showed comparable results to that on the titanium. The mRNA value of on the zirconia was higher than that on the titanium at day four. The protein level of was expressed thicker on the zirconia when compared to the titanium at day two. The results of this study suggest that zirconia material provides adequate osteoclastogenesis behaviors for dental implant use.
作为牙科钛植入物的替代品，氧化锆值得研究，以克服钛的缺点。本研究调查了氧化锆植入材料的表面特性以及与钛相比其表面上的破骨细胞生成反应。制备了5%氧化钇稳定的四方多晶氧化锆标本，并将破骨细胞前体细胞在标本上培养并分化为破骨细胞。评估了表面形状、粗糙度和化学成分。培养后，使用抗酒石酸酸性磷酸酶活性（TRACP）分析细胞形态和分化能力。测量了两个关键转录因子，活化T细胞核因子1（）和的mRNA，并研究了和的蛋白质水平。氧化锆标本呈菱形，表面光滑，与钛标本相比，表面粗糙度无差异。两种材料上分化破骨细胞的形态相似。氧化锆上的TRACP活性与钛上的结果相当。在第4天，氧化锆上的mRNA值高于钛上的。在第2天，与钛相比，氧化锆上的蛋白质水平表达更厚。本研究结果表明，氧化锆材料为牙科植入物的使用提供了足够的破骨细胞生成行为。
