Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.
Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.
Dent Mater. 2020 Jun;36(6):e194-e205. doi: 10.1016/j.dental.2020.04.003. Epub 2020 Apr 30.
The impact of the implant surface material and roughness on inflammatory processes in peri-implantitis is not entirely clear. Hence, we investigated how titanium and zirconia surfaces with different roughness influence the susceptibility of primary human gingival fibroblasts to different inflammatory stimuli.
Primary human gingival fibroblasts were isolated from 8 healthy individuals and cultured on following surfaces: smooth titanium machined surface (TiM), smooth zirconia machined surface (ZrM), moderately rough titanium surface (SLA), or moderately rough zirconia surface (ZLA). Subsequently, stimulation with one of the following stimuli was performed: Porphyromonas gingivalis lipopolysaccharide (LPS), tumor necrosis factor (TNF)-α, interleukin (IL)-1β. The resulting production of IL-6, IL-8, and monocyte chemoattractant protein (MCP)-1 was measured by qPCR and ELISA.
P. gingivalis LPS induced IL-6 and MCP-1 production was slightly higher on titanium surfaces compared to zirconia surfaces. IL-1β induced IL-6 production was not affected by any surface characteristic. The production of MCP-1 in response to IL-1β was higher on smooth compared to rough surfaces and was not affected by the material. The production of IL-6 and MCP-1 in response to TNF-α was most strongly affected by surface characteristics. Higher production of these cytokine was observed on smooth compared to rough surfaces and on titanium compared to zirconia surfaces. Surface characteristics had only minor effects on IL-8 production.
The susceptibility of primary gingival fibroblasts to inflammation depends on various factors, such as surface material, surface roughness and the nature of inflammatory stimuli. All these factors might determine susceptibility to peri-implantitis.
种植体表面材料和粗糙度对种植体周围炎炎症过程的影响尚不完全清楚。因此，我们研究了不同粗糙度的钛和氧化锆表面如何影响原代人牙龈成纤维细胞对不同炎症刺激物的易感性。
从 8 名健康个体中分离原代人牙龈成纤维细胞，并在以下表面上培养：光滑钛机械加工表面（TiM）、光滑氧化锆机械加工表面（ZrM）、中度粗糙钛表面（SLA）或中度粗糙氧化锆表面（ZLA）。然后，用以下刺激物之一进行刺激：牙龈卟啉单胞菌脂多糖（LPS）、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β。通过 qPCR 和 ELISA 测量产生的 IL-6、IL-8 和单核细胞趋化蛋白-1（MCP-1）。
与氧化锆表面相比，牙龈卟啉单胞菌 LPS 诱导的 IL-6 和 MCP-1 产生在钛表面略高。任何表面特性都不会影响 IL-1β 诱导的 IL-6 产生。与粗糙表面相比，光滑表面响应 IL-1β 产生的 MCP-1 更高，并且不受材料影响。响应 TNF-α 产生的 IL-6 和 MCP-1 受表面特性的影响最大。与粗糙表面相比，在光滑表面上观察到这些细胞因子的产生更高，与氧化锆表面相比，在钛表面上观察到更高的产生。表面特性对 IL-8 产生的影响较小。
原代牙龈成纤维细胞对炎症的易感性取决于多种因素，例如表面材料、表面粗糙度和炎症刺激物的性质。所有这些因素都可能决定对种植体周围炎的易感性。
