Lee Seon-Ki, Ji Min-Kyung, Jo Yu-Jin, Park Chan, Cho Hoonsung, Lim Hyun-Pil
Department of Prosthodontics, Daejeon Dental Hospital, Wonkwang University, Daejeon 35233, Korea.
Dental 4D Research Center, Chonnam National University, 33 Yongbong-ro, Buk-gu, Gwangju 61186, Korea.
Materials (Basel). 2022 Aug 3;15(15):5348. doi: 10.3390/ma15155348.
Plasma treatment on a zirconia surface prevents bacterial contamination and maintains osteoblast activity. To assess the degree of adhesion of Porphyromonas gingivalis on a zirconia surface after non-thermal plasma (NTP) treatment, specimens were treated with plasma for 60, 300, and 600 s, after which P. gingivalis was inoculated onto the surface and incubated for 48 h. To assess osteoblast activity after NTP treatment, osteoblasts (MC3T3-E1) were dispensed onto the specimens contaminated with P. gingivalis immediately after NTP for 60 and 120 s, followed by incubation for 48, 72, and 96 h. P. gingivalis was cultured after 60 s of NTP treatment of zirconia. The NTP and control groups showed no significant difference (p = 0.91), but adhesion was significantly increased following NTP treatment for 300 s or longer (300, 600 s groups) (p < 0.05). After NTP treatment of P. gingivalis-contaminated zirconia, osteoblast activity significantly increased at 72 and 96 h (I60 and I120 s group) in the groups treated with plasma (p < 0.017). Application of NTP to dental zirconia implants for 60 s not only inhibits the proliferation of P. gingivalis, which causes peri-implantitis but also increases osseointegration on zirconia surfaces contaminated with P. gingivalis.
氧化锆表面的等离子体处理可防止细菌污染并维持成骨细胞活性。为评估非热等离子体（NTP）处理后牙龈卟啉单胞菌在氧化锆表面的黏附程度，将样本分别用等离子体处理60、300和600秒，之后将牙龈卟啉单胞菌接种到表面并孵育48小时。为评估NTP处理后的成骨细胞活性，在NTP处理60和120秒后，立即将成骨细胞（MC3T3-E1）接种到被牙龈卟啉单胞菌污染的样本上，随后分别孵育48、72和96小时。氧化锆经NTP处理60秒后培养牙龈卟啉单胞菌。NTP组和对照组无显著差异（p = 0.91），但NTP处理300秒及更长时间（300、600秒组）后黏附显著增加（p < 0.05）。在对被牙龈卟啉单胞菌污染的氧化锆进行NTP处理后，血浆处理组在72和96小时时成骨细胞活性显著增加（I60和I120秒组）（p < 0.017）。对牙科氧化锆植入物应用NTP 60秒，不仅可抑制导致种植体周围炎的牙龈卟啉单胞菌的增殖，还可增强在被牙龈卟啉单胞菌污染的氧化锆表面的骨整合。
