Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.
Interdepartmental Research Center "Nutraceuticals and Food for Health", University of Pisa, via del Borghetto 80, 56124 Pisa, Italy.
Molecules. 2019 Mar 4;24(5):900. doi: 10.3390/molecules24050900.
Salmonella enterica serotype Enteritidis and S. enterica serotype Typhimurium are frequently present among poultry and are associated with outbreaks of human salmonellosis. The study investigated the in vitro antimicrobial activity of essential oils (EOs) obtained from Aloysia triphylla, Cinnamomum zeylanicum, Cymbopogon citratus, Litsea cubeba, Mentha piperita, Syzygium aromaticum against S. Enteritidis and S. Thyphimurium strains previously isolated from poultry. A 1:1 mixture of C. zeylanicum and S. aromaticum was also tested. The activity of all compounds was evaluated against the yeast Saccharomyces cerevisiae, commonly used as probiotic. The highest antibacterial activity was observed for C. zeylanicum (minimum inhibitory concentrations (MICs) ranging from 1.26 mg/mL to 0.63 mg/mL), S. aromaticum (MICs from 2.637 mg/mL to 0.164 mg/mL) and the mixture (MICs from 1.289 mg/mL to 0.322 mg/mL). No activity was recorded against S. cerevisiae. The results suggest a possible use of C. zeylanicum and S. aromaticum, alone or in combination, in the farm environment for disinfection and in poultry diet, combined with S. cerevisiae administration, for an integrated approach to avoid Salmonella intestinal colonization.
肠炎沙门氏菌血清型肠炎和鼠伤寒沙门氏菌经常存在于家禽中，与人类沙门氏菌病的爆发有关。本研究调查了从三叶香桃木、肉桂、香茅、山苍子、薄荷、丁香中提取的精油（EOs）对先前从家禽中分离出的肠炎沙门氏菌和鼠伤寒沙门氏菌菌株的体外抗菌活性。还测试了肉桂和丁香的 1:1 混合物。所有化合物的活性均针对用作益生菌的酵母酿酒酵母进行了评估。肉桂（最低抑菌浓度（MIC）范围为 1.26 毫克/毫升至 0.63 毫克/毫升）、丁香（MIC 范围为 2.637 毫克/毫升至 0.164 毫克/毫升）和混合物（MIC 范围为 1.289 毫克/毫升至 0.322 毫克/毫升）表现出最高的抗菌活性。对酿酒酵母没有活性记录。结果表明，肉桂和丁香单独或联合使用，在农场环境中用于消毒，以及在禽类饮食中与酿酒酵母联合使用，可能是一种综合方法，以避免沙门氏菌肠道定植。
