BioArmor S.A., Z.I de la Gare, F-22940 Plaintel, France.
Laboratoire de Biotechnologie et Chimie Marines EA 3884, Université Bretagne Sud, F-56100 Lorient, France.
Molecules. 2021 Sep 15;26(18):5598. doi: 10.3390/molecules26185598.
The regulation of intestinal colonization in livestock by means of non-bactericidal additives is an important management lever for zoonotic bacteria such as spp. is proposed here as a model for the evaluation of five essential oils (EOs) as anti-colonization products against Typhimurium. An evaluation of the toxicity of EOs for showed LD values ranging from 74.5 ± 9.6 µg/mL for (CEO) to 271.6 ± 14.9 µg/mL for (SyEO). Both EOs significantly inhibited bacterial colonization in the digestive tract of with reductions of 0.88 and 0.70 log CFU/nematode at nontoxic concentrations of 50 µg/mL and 150 µg/mL, respectively. With the minimal bactericidal concentrations of CEO and SyEO against Typhimurium being 312.5 µg/mL and 625 µg/mL, respectively, an antibacterial effect can be excluded to explain the inhibition of the bacterial load. The anti-colonizing activity of these two EOs could, however, be related to an inhibition of the swimming motility, which was significantly reduced by 23.47% for CEO at 50 µg/mL and 19.56% for SyEO at 150 µg/mL. This study shows the potential of as a predictive in vivo model of anti-colonizing activities that is suitable for the evaluation of essential oils.
通过非杀菌添加剂调节家畜肠道定植是防控 spp. 等动物源致病菌的重要管理手段。本研究以 Typhimurium 为模型，评估了 5 种精油（EOs）作为抗定植产品的效果。结果显示，对于 ，EOs 的毒性 LD 值范围为 74.5 ± 9.6 µg/mL（CEO）至 271.6 ± 14.9 µg/mL（SyEO）。两种 EO 在非毒性浓度 50 µg/mL 和 150 µg/mL 时，分别显著抑制了肠道内细菌的定植，使细菌载量减少了 0.88 和 0.70 log CFU/线虫。CEO 和 SyEO 对 Typhimurium 的最小杀菌浓度分别为 312.5 µg/mL 和 625 µg/mL，因此抑菌作用可以排除，说明抑制细菌负荷的原因并非杀菌作用。然而，这两种 EO 的抗定植活性可能与抑制游动能力有关，在 50 µg/mL 的 CEO 和 150 µg/mL 的 SyEO 时，游动能力分别显著降低了 23.47%和 19.56%。本研究表明， 可作为抗定植活性的预测性体内模型，适用于评估精油。
