Depaertment of Chemical Engineering & Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen, 361021, China.
School of Marine Engineering, Jimei University, Xiamen, 361021, China.
Sci Rep. 2019 Mar 5;9(1):3489. doi: 10.1038/s41598-019-40209-1.
Carbon nanoparticles (CNPs) have been combined with aptamer, providing a broad application in small molecule. CNPs can be quenched by small molecules and are usually applied as luminescent probes because of their photophysical characteristics. In this work, we developed a competitive analysis for antibiotic residues detection based on carbon nanoparticles (CNPs) and oligonucleotide probes. Oligonucleotide probes including oxytetracycline (OTC) aptamer was exploited for recognition OTC and was used to restore the luminescence. Tetracycline (TC), as a competitor of OTC, was utilized to quench the luminescence of CNPs and reduce the sample matrix effect. Under optimal conditions, the linear rang of OTC was 0.010~1.0 ng/mL with the relative standard deviations (RSDs) from 2.91% to 11.3%, and the limit of detection (LOD) was low to 0.002 ng/mL. Moreover, the proposal was successfully applied to analyze OTC from drink water, indicating that this approach has great potential for other small molecule analysis.
碳纳米粒子 (CNPs) 已与适体结合，为小分子提供了广泛的应用。由于其光物理特性，CNPs 可以被小分子猝灭，通常用作荧光探针。在这项工作中，我们开发了一种基于碳纳米粒子 (CNPs) 和寡核苷酸探针的抗生素残留检测竞争分析方法。包括土霉素 (OTC) 适体的寡核苷酸探针被用于识别 OTC，并用于恢复发光。四环素 (TC) 作为 OTC 的竞争物，用于猝灭 CNPs 的发光并降低样品基质效应。在最佳条件下，OTC 的线性范围为 0.010-1.0ng/mL，相对标准偏差 (RSD) 为 2.91%至 11.3%，检测限 (LOD) 低至 0.002ng/mL。此外，该方法成功应用于饮用水中 OTC 的分析，表明该方法在其他小分子分析中具有很大的潜力。
