抗菌剂无限抑制浓度的定量测定。
Quantitative determination of infinite inhibition concentrations of antimicrobial agents.
作者信息
Marwan A G, Nagel C W
出版信息
Appl Environ Microbiol. 1986 Mar;51(3):559-61. doi: 10.1128/aem.51.3.559-561.1986.
We developed a method to determine the infinite inhibition concentrations (IICs) of antimicrobial agents. This method was based on finding the relative effectiveness of an inhibitor at various concentrations. Benzoic acid and parabens were tested on Saccharomyces bayanus, Hansenula sp., and Pseudomonas fluorescens. The relative effectiveness values of these compounds were established. A plot of the inhibitor concentration versus the reciprocal of relative effectiveness was linear. The chi-axis intercept was the concentration of the inhibitor which gave infinite microbial inhibition. For S. bayanus the IICs were 330, 930, 480, and 220 ppm (330, 930, 480, and 220 ml/liter) for benzoic acid and methyl-, ethyl-, and propylparabens, respectively. For Hansenula sp. the IIC was 180 ppm for benzoic acid. For P. fluorescens the IICs were 1,310, 960, and 670 ppm for methyl-, ethyl-, and propylparabens, respectively. Our results indicated that the IIC is affected by the growth medium. The advantages and applications of this method are discussed.
我们开发了一种测定抗菌剂无限抑制浓度（IICs）的方法。该方法基于确定抑制剂在不同浓度下的相对有效性。对巴氏酵母、汉逊酵母属和荧光假单胞菌测试了苯甲酸和对羟基苯甲酸酯。确定了这些化合物的相对有效性值。抑制剂浓度与相对有效性倒数的关系图呈线性。x轴截距即为产生无限微生物抑制的抑制剂浓度。对于巴氏酵母，苯甲酸和对羟基苯甲酸甲酯、乙酯和丙酯的IICs分别为330、930、480和220 ppm（330、930、480和220 ml/升）。对于汉逊酵母属，苯甲酸的IIC为180 ppm。对于荧光假单胞菌，对羟基苯甲酸甲酯、乙酯和丙酯的IICs分别为1310、960和670 ppm。我们的结果表明IIC受生长培养基的影响。讨论了该方法的优点和应用。
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