Scott J G, Georghiou G P
Biochem Genet. 1986 Feb;24(1-2):25-37. doi: 10.1007/BF00502976.
Permethrin resistance in the Learn-PyR strain of house fly was examined in four genetically derived substrains, each being homozygous for a different "resistant" autosome of the Learn-PyR strain. The resistance of these derivative strains was characterized toxicologically and biochemically. The relative levels of resistance to permethrin conferred by each autosome were 5 greater than 3 greater than 1 greater than 2. Three factors were associated with resistance: (1) increased mixed-function oxidase (MFO) activity associated with elevated levels of cytochrome P-450, cytochrome b5, and NADPH-cytochrome c reductase (P-450 reductase) activity; (2) target-site insensitivity (kdr); and (3) decreased cuticular penetration. Permethrin resistance factors on chromosome 1 consisted of a piperonyl butoxide (PB)-suppressible mechanism correlated with increased levels of cytochromes P-450 and b5; on chromosome 2, a PB-suppressible mechanism associated with elevated amounts of cytochrome P-450; on chromosome 3, decreased cuticular penetration, kdr, and increased amounts of P-450 reductase activity; and on chromosome 5, a largely PB-suppressible mechanism correlated with elevated levels of cytochrome P-450 and P-450 reductase activity.
在家蝇的Learn - PyR品系的四个遗传衍生亚系中检测了对氯菊酯的抗性，每个亚系对于Learn - PyR品系的不同“抗性”常染色体都是纯合的。对这些衍生菌株的抗性进行了毒理学和生物化学特征分析。每个常染色体赋予的对氯菊酯的相对抗性水平为5大于3大于1大于2。有三个因素与抗性相关：（1）混合功能氧化酶（MFO）活性增加，与细胞色素P - 450、细胞色素b5和NADPH - 细胞色素c还原酶（P - 450还原酶）活性水平升高有关；（2）靶位点不敏感（kdr）；（3）表皮穿透性降低。1号染色体上的氯菊酯抗性因子包括一种胡椒基丁醚（PB）可抑制的机制，与细胞色素P - 450和b5水平升高相关；2号染色体上，一种与细胞色素P - 450含量升高相关的PB可抑制机制；3号染色体上，表皮穿透性降低、kdr以及P - 450还原酶活性增加；5号染色体上，一种主要由PB可抑制的机制，与细胞色素P - 450和P - 450还原酶活性水平升高相关。
