Liu N, Tomita T, Scott J G
Department of Entomology, New York 14853-0901.
Experientia. 1995 Feb 15;51(2):164-7. doi: 10.1007/BF01929363.
A cytochrome P450, termed P450lpr, is the major P450 responsible for pyrethroid resistance in the Learn-PyR (LPR) strain of house fly. Recently, the putative gene (CYP6D1) coding for P450lpr has been sequenced from the LPR and aabys strains of house fly. Allele-specific polymerase chain reaction (ASPCR) was used for linkage group analysis with backcross progeny from the wild type LPR strain and a multiple marker strain (aabys). We found that CYP6D1 is linked to chromosome 1. The possible role of regulatory or modifying genes responsible for elevated P450lpr expression is discussed in relation to the chromosomal linkage of CYP6D1.
一种名为P450lpr的细胞色素P450，是家蝇Learn-PyR（LPR）品系中对拟除虫菊酯产生抗性的主要P450。最近，已从家蝇的LPR品系和aabys品系中对编码P450lpr的推定基因（CYP6D1）进行了测序。等位基因特异性聚合酶链反应（ASPCR）用于对野生型LPR品系和多标记品系（aabys）的回交后代进行连锁群分析。我们发现CYP6D1与1号染色体连锁。结合CYP6D1的染色体连锁情况，讨论了负责P450lpr表达升高的调控或修饰基因的可能作用。
