Hsiao June, Wang Yuanyuan, Zheng Li, Liu Ruirui, Said Raed, Hadjiyski Lubomir, Cha Heekon, Botero Tatiana, Chatzistavrou Xanthippi, Dong Qing, Papagerakis Silvana, Papagerakis Petros
Department of Orthodontics and Pediatric Dentistry, School of Dentistry, University of Michigan, Ann Arbor, MI, USA.
Dept. of Prosthodontics, School & Hospital of Stomatology, Xi'an Jiaotong University, Jiaotong, China.
Methods Mol Biol. 2019;1922:393-403. doi: 10.1007/978-1-4939-9012-2_35.
Dental caries is an infectious oral disease caused primarily by complex interactions of cariogenic oral flora (biofilm) with dietary carbohydrates on the tooth surface over time. Streptococcus mutans and Streptococcus sobrinus (S. mutans and S. sobrinus) are the most prevalent cariogenic species within the oral biofilm and considered the main etiological agents of caries. Pulp exposure and infection can be caused by trauma, carious lesion, and mechanical reasons. Pulp response to these exposures depends on the state of the pulp as well as the potential bacterial contamination of pulp tissue. Herein, we describe the process of using two in vivo rodent models to study the progression of dental caries and pulp disease: a nutritional microbial model and a pulp disease induction model. The progression of the carious lesion and pulpal infections in both models was assessed by micro-CT imaging and histomorphometric analysis. Moreover, the pulp disease induction models can be used to compare and assess the antibacterial and reparative properties of the different pulp capping materials.
龋齿是一种主要由致龋性口腔菌群（生物膜）与饮食中的碳水化合物在牙齿表面长期复杂相互作用引起的感染性口腔疾病。变形链球菌和远缘链球菌是口腔生物膜中最常见的致龋菌，被认为是龋齿的主要病因。牙髓暴露和感染可由外伤、龋损及机械原因引起。牙髓对这些暴露的反应取决于牙髓的状态以及牙髓组织潜在的细菌污染情况。在此，我们描述了使用两种体内啮齿动物模型来研究龋齿和牙髓疾病进展的过程：营养微生物模型和牙髓疾病诱导模型。通过显微计算机断层扫描成像和组织形态计量分析评估了两种模型中龋损和牙髓感染的进展情况。此外，牙髓疾病诱导模型可用于比较和评估不同牙髓盖髓材料的抗菌和修复性能。
